Yazdanbakhsh M, Eckmann C M, Roos D
J Immunol. 1985 Aug;135(2):1378-84.
The interaction of human eosinophils with opsonized particles was compared with that of human neutrophils. When eosinophils are stimulated with serum-opsonized zymosan particles, the lag time in H2O2 production is twice as long as found with neutrophils. Moreover, the concentration of these IgG + C3-coated particles required for optimal stimulation is about four times as high for eosinophils as for neutrophils. Under these conditions, the two cell types generate similar amounts of H2O2. However, eosinophils produce twice as much H2O2 as do neutrophils when stimulated with the soluble agent phorbol myristate acetate. Thus, although the oxidase capacity of eosinophils is larger than that of neutrophils, opsonized zymosan is a weak trigger for this activity in eosinophils. This phenomenon may be due to differences between the two cell types in the plasma membrane receptors or in the receptor oxidase transducing signal. The following are indications for the first possibility. i) IgG interacts poorly with the Fc gamma receptors on the eosinophil surface compared with those on neutrophils. This was shown by the inability of IgG-coated zymosan or IgG-coated latex to trigger any substantial H2O2 production by eosinophils unless brought into close contact with these cells by centrifugation. In contrast, neutrophils are stimulated by these particles both in suspension and in a pellet. The dissimilarity of the Fc gamma receptors on eosinophils and neutrophils was also shown with respect to antigenicity, determined by the monoclonal antibodies 3G8 and CLB-FcR-1. ii) Eosinophils contain about half as many receptors for C3b and C3bi on their surface as do neutrophils, also detected with monoclonal antibodies. The interaction of IgG subclasses with functional Fc gamma receptors on eosinophils and neutrophils showed that eosinophils release twice as much H2O2 as do neutrophils upon interaction with IgG1-, IgG2-, or IgG3-coated Sepharose beads, but this difference becomes fivefold with IgG4-coated Sepharose. This might be of relevance to the situation of chronic antigenic stimulation, e.g., in chronic schistosomiasis, in which eosinophil numbers and IgG4 antibody levels are elevated.
将人类嗜酸性粒细胞与调理素化颗粒的相互作用与人类中性粒细胞的相互作用进行了比较。当用血清调理素化的酵母聚糖颗粒刺激嗜酸性粒细胞时，过氧化氢产生的延迟时间是中性粒细胞的两倍。此外，嗜酸性粒细胞达到最佳刺激所需的这些IgG + C3包被颗粒的浓度约为中性粒细胞的四倍。在这些条件下，两种细胞类型产生的过氧化氢量相似。然而，当用可溶性试剂佛波醇肉豆蔻酸酯刺激时，嗜酸性粒细胞产生的过氧化氢量是中性粒细胞的两倍。因此，尽管嗜酸性粒细胞的氧化酶能力大于中性粒细胞，但调理素化的酵母聚糖对嗜酸性粒细胞的这种活性是一种较弱的触发因素。这种现象可能是由于两种细胞类型在质膜受体或受体氧化酶转导信号方面存在差异。以下是第一种可能性的迹象。i）与中性粒细胞相比，IgG与嗜酸性粒细胞表面的Fcγ受体相互作用较差。这表现为IgG包被的酵母聚糖或IgG包被的乳胶无法触发嗜酸性粒细胞产生任何大量的过氧化氢，除非通过离心使其与这些细胞紧密接触。相比之下，中性粒细胞在悬浮液和沉淀中都受到这些颗粒的刺激。嗜酸性粒细胞和中性粒细胞上Fcγ受体在抗原性方面的差异也通过单克隆抗体3G8和CLB - FcR - 1得以体现。ii）嗜酸性粒细胞表面C3b和C3bi的受体数量约为中性粒细胞的一半，这也通过单克隆抗体检测到。IgG亚类与嗜酸性粒细胞和中性粒细胞上功能性Fcγ受体的相互作用表明，与IgG1 - 、IgG2 - 或IgG3包被的琼脂糖珠相互作用时，嗜酸性粒细胞释放的过氧化氢量是中性粒细胞的两倍，但与IgG4包被的琼脂糖珠相互作用时，这种差异变为五倍。这可能与慢性抗原刺激的情况有关，例如在慢性血吸虫病中，嗜酸性粒细胞数量和IgG4抗体水平会升高。
