从人类分离出的沙门氏菌菌株无细胞提取物中的肠毒素、细胞毒素、坏死和致死活性。

Enterotoxic, cytotoxic, necrotic and lethal activities in cell-free extracts of Salmonella strains isolated from humans.

作者信息

González E A, Blanco J, Blanco M, Garabal J I, Alonso M P

机构信息

Departamento de Microbiología y Parasitología, Facultad de Veterinaria, Universidad de Santiago de Compostela, Lugo, Spain.

出版信息

Zentralbl Bakteriol. 1989 Sep;271(3):281-92. doi: 10.1016/s0934-8840(89)80026-x.

DOI:10.1016/s0934-8840(89)80026-x

PMID:2803456

Abstract

Unconcentrated cell-free sonic extracts from thirty Salmonella strains isolated from the faeces and blood of humans were investigated for the production of enterotoxins in various tests (Vero cell, infant mouse, rabbit skin permeability and rabbit ileal loop), as well as for lethal activity in adult mice. Sonic extracts from 23 (76.7%) strains were lethal for mice, 21 (70%) increased skin permeability and 3 (10%) showed necrotizing activity for the rabbit skin. No Salmonella strain producing typical Escherichia coli toxins, such as thermolabile (LT) or thermostable (STa) enterotoxins, Verotoxin (VT) or cytotoxic necrotizing factor (CNF) cytotoxins, were detected. Non-repetitive fluid accumulation in rabbit loops was obtained when unconcentrated sonic extracts from 10 selected strains were assayed in seven rabbits. Growth of Salmonella in casamino acid yeast extract medium, followed by treatment of bacterial cells with polymyxin B, was demonstrated to be a rapid and sensitive method for releasing the delayed permeability factor.

摘要

对从人类粪便和血液中分离出的30株沙门氏菌的未浓缩无细胞超声提取物进行了研究，检测其在各种试验（Vero细胞、幼鼠、兔皮肤通透性和兔回肠袢试验）中产生肠毒素的情况，以及对成年小鼠的致死活性。23株（76.7%）菌株的超声提取物对小鼠具有致死性，21株（70%）可增加皮肤通透性，3株（10%）对兔皮肤表现出坏死活性。未检测到产生典型大肠杆菌毒素（如不耐热（LT）或耐热（STa）肠毒素、维罗毒素（VT）或细胞毒性坏死因子（CNF）细胞毒素）的沙门氏菌菌株。当对7只兔子检测10株选定菌株的未浓缩超声提取物时，在兔肠袢中获得了非重复性液体蓄积。结果表明，在酪蛋白氨基酸酵母提取物培养基中培养沙门氏菌，然后用多粘菌素B处理细菌细胞，是释放延迟通透性因子的一种快速且灵敏的方法。