产志贺毒素大肠杆菌菌株的培养上清液可引起兔回肠袢积液。

Culture supernatant of Shiga toxin-producing Escherichia coli strains provoke fluid accumulation in rabbit ileal loops.

作者信息

Ferreira A J, Elias W P, Pelayo J S, Giraldi R, Pedroso M Z, Scaletsky I C

机构信息

Departamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Brazil.

出版信息

FEMS Immunol Med Microbiol. 1997 Dec;19(4):285-8. doi: 10.1111/j.1574-695X.1997.tb01098.x.

DOI:10.1111/j.1574-695X.1997.tb01098.x

PMID:9537753

Abstract

Production of Shiga toxin (Stx) in Escherichia coli strains belonging to serogroups O26, O111, and O157 was evaluated in the rabbit ileal loop assay and results were compared to those using tissue culture assays and DNA hybridization with specific probes for Stx1 and Stx2. All 14 Shiga toxin-producing E. coli strains tested provoked fluid accumulation in the rabbit intestinal loop. Eleven strains hybridized with Stx1 probe, one strain with Stx2 and two strains with both probes. Filtered culture supernatants of all E. coli strains presented cytotoxic effects in both HeLa and Vero cells. In this study, we found a strong association between the production of Stx and its effect in an animal model. This is the first description of high-level Stx-producing E. coli O111ac isolated in Brazil.

摘要

采用兔回肠袢试验评估了血清群O26、O111和O157的大肠杆菌菌株中志贺毒素（Stx）的产生情况，并将结果与使用组织培养试验以及用针对Stx1和Stx2的特异性探针进行DNA杂交的结果进行了比较。所测试的14株产志贺毒素大肠杆菌菌株均在兔肠袢中引起液体蓄积。11株与Stx1探针杂交，1株与Stx2探针杂交，2株与两种探针均杂交。所有大肠杆菌菌株的过滤培养上清液在HeLa细胞和Vero细胞中均呈现细胞毒性作用。在本研究中，我们发现Stx的产生与其在动物模型中的效应之间存在密切关联。这是在巴西分离出的高产志贺毒素大肠杆菌O111ac的首次描述。