Ayala Yareni A, Pontes Bruno, Hissa Barbara, Monteiro Ana Carolina M, Farina Marcos, Moura-Neto Vivaldo, Viana Nathan B, Nussenzveig H Moysés
LPO-COPEA, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil; Instituto de Física, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-972, Brazil.
LPO-COPEA, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil.
Exp Cell Res. 2017 Feb 15;351(2):173-181. doi: 10.1016/j.yexcr.2016.12.016. Epub 2016 Dec 27.
Mechanical properties of cells are known to be influenced by the actin cytoskeleton. In this article, the action of drugs that interact with the actin cortex is investigated by tether extraction and rheology experiments using optical tweezers. The influences of Blebbistatin, Cytochalasin D and Jasplakinolide on the cell mechanical properties are evaluated. The results, in contradiction to current views for Jasplakinolide, show that all three drugs and treatments destabilize the actin cytoskeleton, decreasing the cell membrane tension. The cell membrane bending modulus increased when the actin cytoskeleton was disorganized by Cytochalasin D. This effect was not observed for Blebbistatin and Jasplakinolide. All drugs decreased by two-fold the cell viscoelastic moduli, but only Cytochalasin D was able to alter the actin network into a more fluid-like structure. The results can be interpreted as the interplay between the actin network and the distribution of myosins as actin cross-linkers in the cytoskeleton. This information may contribute to a better understanding of how the membrane and cytoskeleton are involved in cell mechanical properties, underlining the role that each one plays in these properties.
已知细胞的力学性质会受到肌动蛋白细胞骨架的影响。在本文中，通过使用光镊的系链提取和流变学实验，研究了与肌动蛋白皮层相互作用的药物的作用。评估了肌球蛋白抑制剂、细胞松弛素D和茉莉酸内酯对细胞力学性质的影响。与目前关于茉莉酸内酯的观点相反，结果表明，所有这三种药物和处理都会破坏肌动蛋白细胞骨架的稳定性，降低细胞膜张力。当细胞松弛素D使肌动蛋白细胞骨架紊乱时，细胞膜弯曲模量增加。对于肌球蛋白抑制剂和茉莉酸内酯，未观察到这种效应。所有药物均使细胞粘弹性模量降低了两倍，但只有细胞松弛素D能够将肌动蛋白网络改变为更类似流体的结构。这些结果可以解释为肌动蛋白网络与作为细胞骨架中肌动蛋白交联剂的肌球蛋白分布之间的相互作用。这些信息可能有助于更好地理解膜和细胞骨架如何参与细胞力学性质，强调了它们各自在这些性质中所起的作用。
