Nakano Shota, Yamamoto Shohei, Okada Atsumasa, Nakajima Tadaaki, Sato Mamiko, Takagi Tomoko, Tomooka Yasuhiro
Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan.
Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan; Graduate Program in Bioscience, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan.
Biochem Biophys Res Commun. 2017 Jan 29;483(1):245-251. doi: 10.1016/j.bbrc.2016.12.158. Epub 2016 Dec 26.
Extracellular vesicles (EVs) have been shown to transport miRNA, mRNA and protein, suggesting that they are new communication mediators. Diffusible mesenchymal factors determine the fate of Műllerian epithelial cells into oviductal ciliated cells. In the present study, we investigated whether EVs mediate the communication in the epithelial-mesenchymal interaction during oviductal ciliogenesis. EVs were isolated from cells of oviductal mesenchymal cell line (S1 cells) and characterized by TEM and expression of exosomal marker CD81. CD81 protein was also detected in oviductal mesenchyme, suggesting that CD81-expressing exosomes may be secreted from oviductal mesenchyme, as well as S1 cells. β-actin, Gapdh and Vimentin mRNAs and miRNAs were detected in the exosomes. mRNA in S1 cells was able to be transported into cells of Műllerian epithelial cell line (E1 cells) via the exosomes. The effects of exosomes derived from S1 cells on ciliogenesis of E1 cells were analyzed by in vitro models. Culture with exosomes increased the number of ciliated cells in E1 cells. These results suggest that exosomes derived from mesenchymal cells modulate the oviductal ciliogenesis and open new avenues for developmental study of EVs.
细胞外囊泡（EVs）已被证明可运输微小RNA（miRNA）、信使核糖核酸（mRNA）和蛋白质，这表明它们是新的通讯介质。可扩散的间充质因子决定了苗勒管上皮细胞向输卵管纤毛细胞的命运。在本研究中，我们调查了细胞外囊泡是否在输卵管纤毛发生过程中介导上皮-间充质相互作用中的通讯。从输卵管间充质细胞系（S1细胞）中分离出细胞外囊泡，并通过透射电子显微镜（TEM）和外泌体标志物CD81的表达进行表征。在输卵管间充质中也检测到了CD81蛋白，这表明表达CD81的外泌体可能由输卵管间充质以及S1细胞分泌。在外泌体中检测到了β-肌动蛋白、甘油醛-3-磷酸脱氢酶（Gapdh）和波形蛋白的mRNA和miRNA。S1细胞中的mRNA能够通过外泌体转运到苗勒管上皮细胞系（E1细胞）中。通过体外模型分析了S1细胞来源的外泌体对E1细胞纤毛发生的影响。用外泌体培养增加了E1细胞中纤毛细胞的数量。这些结果表明，间充质细胞来源的外泌体调节输卵管纤毛发生，并为细胞外囊泡的发育研究开辟了新途径。
