Mai Weijun, Huang Fang, Chen Huiqing, Zhou Yajing, Chen Yan
The Institute of Life Sciences, Jiangsu University, Zhenjiang, 212013, China.
The Institute of Life Sciences, Jiangsu University, Zhenjiang, 212013, China.
Virus Res. 2017 Feb 15;230:1-6. doi: 10.1016/j.virusres.2016.12.015. Epub 2016 Dec 26.
Nucleolar proteins facilitate the replication of certain human and animal viruses through interaction with viral proteins. In this study, an interaction between nervous necrosis virus capsid protein and nucleolar phosphoprotein B23 was identified using in vitro experimental approaches. The capsid protein binds to B23 early during the viral infection and accumulates in the nucleus, particularly in the nucleolus. However, over the course of the infection B23 is redistributed from the nucleoli to the nucleoplasm. siRNA-mediated knockdown of B23 reduced viral replication and cytopathic effect. Thus, B23 targets capsid protein to the nucleus and facilitates NNV replication. The results provide the first demonstration that nucleolar protein B23 has a direct role in the nodavirus replication process.
核仁蛋白通过与病毒蛋白相互作用促进某些人类和动物病毒的复制。在本研究中，使用体外实验方法鉴定了神经坏死病毒衣壳蛋白与核仁磷蛋白B23之间的相互作用。衣壳蛋白在病毒感染早期与B23结合，并在细胞核中积累，特别是在核仁中。然而，在感染过程中，B23从核仁重新分布到核质中。siRNA介导的B23敲低减少了病毒复制和细胞病变效应。因此，B23将衣壳蛋白靶向细胞核并促进神经坏死病毒的复制。这些结果首次证明核仁蛋白B23在诺达病毒复制过程中具有直接作用。
