Parrinello Daniela, Sanfratello Maria Antonietta, Vizzini Aiti, Testasecca Lelia, Parrinello Nicolò, Cammarata Matteo
Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche, Via Archirafi 18, Palermo, Italy.
Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche, Via Archirafi 18, Palermo, Italy.
Fish Shellfish Immunol. 2017 Mar;62:24-30. doi: 10.1016/j.fsi.2016.12.027. Epub 2016 Dec 26.
The transcription of two Ciona intestinalis galectin genes (CiLgals-a and CiLgals-b) is uparegulated by LPS in the pharynxis (hemocytes, vessel epithelium, endostilar zones) which is retained the main organ of the immunity. In this ascidian, for the first time we show, by immunohistochemistry and in situ hybridization methods, that these two immune-related genes are expressed in the gastric epithelium of naïve ascidians, whereas the galectins appear to be only contained in the intestine columnar epithelium. In addition, according to previous results on the pharynx, the genes are also expressed and galectins produced by hemocytes scattered in the connective tissue surrounding the gut. The genes expression and galectin localization in several tissues, including the previous findings on the transcription upregulation, the constitutive expression of these genes by endostylar zones and by the gastric epithelium suggest a potential multifunctional role of these galectins. In this respect, it is of interest to define where the CiLgals are normally found as related to the tissue functions. Such an approach should be a starting point for further investigations.
两种海鞘（Ciona intestinalis）半乳糖凝集素基因（CiLgals-a和CiLgals-b）的转录在咽部（血细胞、血管上皮、内柱区）被脂多糖上调，咽部是主要的免疫器官。在这种海鞘中，我们首次通过免疫组织化学和原位杂交方法表明，这两个与免疫相关的基因在未受刺激的海鞘的胃上皮中表达，而半乳糖凝集素似乎仅存在于肠柱状上皮中。此外，根据先前关于咽部的研究结果，这些基因也在散在于肠道周围结缔组织中的血细胞中表达并产生半乳糖凝集素。这些基因在多个组织中的表达以及半乳糖凝集素的定位，包括先前关于转录上调的研究结果、这些基因在内柱区和胃上皮中的组成型表达，表明这些半乳糖凝集素具有潜在的多功能作用。在这方面，确定CiLgals与组织功能相关的正常存在位置很有意义。这种方法应该是进一步研究的起点。
