Asonuma K, Tanaka K, Uemoto S, Okamura R, Matsuoka S, Kitakado Y, Utsunomiya H, Katayama T, Tanaka M, Inomata Y
Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Transpl Int. 1989 Oct;2(3):133-6. doi: 10.1007/BF02414599.
Arterial blood ketone body ratio (acetoacetate/3-hydroxybutyrate; KBR), which reflects hepatic mitochondrial redox potential, was measured during a 2-week period after orthotopic liver transplantation in three groups of rats: group 1, the isogenic combination of LEW (RT1l) graft to LEW recipient as control; group 2, the allogenic combination of ACI (RT1a) graft to LEW recipient without immunosuppressive treatment: and group 3, the allogenic combination of ACI to LEW with immunosuppressive treatment using cyclosoporin (CyA). Isogenic recipients survived indefinitely. Allogenic recipients in group 2 had severe rejection with a mean survival of 10.3 +/- 0.54 days, while 77.8% of the allogenic recipients in group 3 survived more than 30 days. KBR of rats surviving more than 2 weeks in groups 1 and 3 gradually increased post-transplantation and was maintained at a high level. By contrast, though KBR in group 2 was restored at 3 days, it gradually fell and remained at a significantly low level (P less than 0.001). It is suggested that KBR provides an accurate indicator for evaluating metabolic viability of the critically deteriorating liver graft accompanied by sever rejection.
动脉血酮体比率（乙酰乙酸/3-羟基丁酸；KBR）反映肝脏线粒体氧化还原电位，在三组大鼠原位肝移植后的2周内进行了测量：第1组，将LEW（RT1l）移植物同基因移植给LEW受体作为对照；第2组，将ACI（RT1a）移植物异体移植给LEW受体且不进行免疫抑制治疗；第3组，将ACI移植物异体移植给LEW受体并使用环孢素（CyA）进行免疫抑制治疗。同基因受体长期存活。第2组的异体受体发生严重排斥反应，平均存活时间为10.3±0.54天，而第3组77.8%的异体受体存活超过30天。第1组和第3组中存活超过2周的大鼠的KBR在移植后逐渐升高并维持在较高水平。相比之下，尽管第2组的KBR在第3天时恢复，但随后逐渐下降并维持在显著较低水平（P<0.001）。提示KBR可为评估伴有严重排斥反应的严重恶化肝移植物的代谢活力提供准确指标。
