Młynarczyk-Bonikowska Beata, Kujawa Marlena, Malejczyk Magdalena, Młynarczyk Grażyna, Majewski Sławomir
Department of Diagnostics of Sexually Transmitted Diseases, Medical University of Warsaw, Warsaw, Poland.
Department of Medical Microbiology, Medical University of Warsaw, Warsaw, Poland.
Postepy Dermatol Alergol. 2016 Dec;33(6):475-479. doi: 10.5114/ada.2016.63887. Epub 2016 Dec 2.
One of two main mechanisms of resistance in tetracycline-resistant (TRNG) is associated with the presence of TetM protein responsible for actively blocking of the tetracycline target site in the 30S ribosomal subunit. This mechanism is encoded by conjugative plasmids. The second mechanism is chromosomal in nature and due to mutations in specific genes.
To determine the incidence and type of determinants in TRNG strains isolated from patients presenting with gonorrhea infection to the Dermatology and Venereology Clinic in Warsaw in 2012-2013.
Tetracycline and doxycycline susceptibility was determined by E-Tests. The presence and type of the gene were determined by polymerase chain reaction.
Tetracycline resistance was detected in 50.8% of the evaluated strains. The TRNG strains containing the plasmid constituted 13.8% of all the evaluated strains. Dutch type constituted 12.3% and American type 1.5% of all the evaluated strains. In the remaining TRNG strains, resistance to tetracyclines was presumably chromosome-encoded. The minimal inhibitory concentration (MIC) of tetracycline ranged from 0.25 to 32.0 mg/l, MIC = 2.0 mg/l, MIC = 32.0 mg/l. The MIC of doxycycline ranged from 0.25 to 32.0 mg/l, MIC = 4.0 mg/l, MIC = 16.0 mg/l.
Unlike most of European countries, in 2012-2013 in Poland, the Dutch type was found to be much more common than the American type. Minimal inhibitory concentration values of tetracycline and doxycycline were similar, with doxycycline exhibiting a somewhat lower effectiveness than tetracycline towards chromosome-mediated tetracycline resistant strains of .
耐四环素淋球菌（TRNG）的两种主要耐药机制之一与TetM蛋白的存在有关，该蛋白负责主动阻断30S核糖体亚基中的四环素靶点。这种机制由接合质粒编码。第二种机制本质上是染色体介导的，是特定基因突变的结果。
确定2012 - 2013年从华沙皮肤病与性病诊所淋病感染患者中分离出的TRNG菌株中耐药决定因素的发生率和类型。
采用E试验测定四环素和多西环素的敏感性。通过聚合酶链反应确定基因的存在和类型。
在50.8%的评估菌株中检测到四环素耐药性。含有质粒的TRNG菌株占所有评估菌株的13.8%。荷兰型占所有评估菌株的12.3%，美国型占1.5%。在其余的TRNG菌株中，对四环素的耐药性可能是由染色体编码的。四环素的最低抑菌浓度（MIC）范围为0.25至32.0mg/l，MIC = 2.0mg/l，MIC = 32.0mg/l。多西环素的MIC范围为0.25至32.0mg/l，MIC = 4.0mg/l，MIC = 16.0mg/l。
与大多数欧洲国家不同，2012 - 2013年在波兰，发现荷兰型比美国型更为常见。四环素和多西环素的最低抑菌浓度值相似，多西环素对染色体介导的四环素耐药菌株的有效性略低于四环素。
