Notarnicola Maria, Tutino Valeria, Tafaro Angela, Bianco Giusy, Guglielmi Emilia, Caruso Maria Gabriella
Laboratory of Nutritional Biochemistry, National Institute for Digestive Diseases "S. de Bellis" , Castellana Grotte, Bari, Italy.
Laboratory of Animal Facility, National Institute for Digestive Diseases "S. de Bellis" , Castellana Grotte, Bari, Italy.
Nutr Healthy Aging. 2016 Oct 27;4(1):73-80. doi: 10.3233/NHA-160008.
Cannabinoid- 2 (CB2) receptor is known for its anti-obesity effects silencing the activated immune cells that are key drivers of metabolic syndrome and inflammation. Nutritional interventions in experimental models of carcinogenesis have been demonstrated to modulate tissue inflammation state and proliferation. Aim of this study was to test, in Apc mice, whether a diet enriched with olive oil, omega- 3 and omega-6- PUFAs affects the adipose tissue inflammation status. Four groups of animal were studied: ST group, receiving a standard diet; OO group, receiving the standard diet in which soybean oil (source of fats) was replaced with olive oil; OM-3 group, receiving the standard diet in which soybean oil was replaced with salmon oil; OM-6 group, receiving the standard diet in which soybean oil was replaced with oenothera oil. Gene and protein expression, in adipose tissue, were evaluated by RT-PCR and Western Blotting, respectively. Enzymatic activities were assayed by fluorescent and radiometric method, where appropriated. The diet enriched with olive oil significantly induced CB2 receptor expression and it was able to control inflammatory and proliferative activity of mice adipose tissue. The present findings open opportunities for developing novel nutritional strategies considering olive oil a key ingredient of a healthy dietary pattern.
大麻素-2（CB2）受体因其抗肥胖作用而闻名，它能使激活的免疫细胞失活，而这些免疫细胞是代谢综合征和炎症的关键驱动因素。在致癌实验模型中的营养干预已被证明可调节组织炎症状态和增殖。本研究的目的是在Apc小鼠中测试富含橄榄油、ω-3和ω-6多不饱和脂肪酸的饮食是否会影响脂肪组织炎症状态。研究了四组动物：ST组，接受标准饮食；OO组，接受用橄榄油替代大豆油（脂肪来源）的标准饮食；OM-3组，接受用鲑鱼油替代大豆油的标准饮食；OM-6组，接受用月见草油替代大豆油的标准饮食。分别通过逆转录聚合酶链反应（RT-PCR）和蛋白质印迹法评估脂肪组织中的基因和蛋白质表达。在适当的情况下，通过荧光和放射测量法测定酶活性。富含橄榄油的饮食显著诱导了CB2受体的表达，并且能够控制小鼠脂肪组织的炎症和增殖活性。本研究结果为开发新的营养策略提供了机会，将橄榄油视为健康饮食模式的关键成分。
