Das Moushumee, Kumar Sachin
Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India.
Arch Virol. 2017 Apr;162(4):997-1007. doi: 10.1007/s00705-016-3182-3. Epub 2016 Dec 29.
Despite the prevalence of Newcastle disease virus (NDV) outbreaks in India through the decades, there has been little genetic characterisation of the virulent strains circulating in Northeast India. In 2014, a poultry farm in the Kamrup district of Assam reported an ND outbreak. In this study, genetic analysis and clinicopathological tests showed the virulent nature of the isolate Kamrup. Based on prudent classification criteria, the virulent strain Kamrup was found to be most closely related to members of genotype XIII of class II NDV. A phylogenetic analysis of NDV strains suggested three sub-genotypes: XIIIa, XIIIb and XIIIc. NDV strain Kamrup belonged to sub-genotype XIIIc. Sub-genotype XIIIc isolates were similar to the 1982 isolate from cockatoo and appeared to have evolved parallel to the preceding genotype XIII viruses circulating in India. The high genetic diversity and frequency of mutations observed in the envelope glycoproteins of strain Kamrup demonstrate the evolution of the pandemic genotype XIII NDV in India, which further undermines and complicates of NDV management in India.
尽管数十年来新城疫病毒（NDV）在印度频繁爆发，但印度东北部地区流行的强毒株的基因特征研究却很少。2014年，阿萨姆邦卡姆鲁普区的一个家禽养殖场报告了新城疫疫情。在本研究中，基因分析和临床病理测试表明了卡姆鲁普分离株的强毒性质。根据审慎的分类标准，发现强毒株卡姆鲁普与II类NDV的基因型XIII成员关系最为密切。对NDV毒株的系统发育分析表明存在三个亚基因型：XIIIa、XIIIb和XIIIc。NDV毒株卡姆鲁普属于亚基因型XIIIc。亚基因型XIIIc分离株与1982年从凤头鹦鹉分离出的毒株相似，似乎与之前在印度流行的基因型XIII病毒平行进化。在卡姆鲁普毒株包膜糖蛋白中观察到的高遗传多样性和突变频率表明了大流行基因型XIII NDV在印度的进化，这进一步破坏了印度NDV的管理并使其复杂化。
