Mohammadiazar Sirwan, Hasanli Fateme, Maham Mehdi, Payami Samarin Somayeh
Department of Chemistry, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran.
Department of Chemistry, Islamic Azad University, Tehran North Branch, Tehran, Iran.
Biomed Chromatogr. 2017 Aug;31(8). doi: 10.1002/bmc.3926. Epub 2017 Jan 30.
Electrochemically co-deposited sol-gel/Cu nanocomposites have been introduced as a novel, simple and single-step technique for preparation of solid-phase microextraction (SPME) coating to extract methadone (MDN) (a synthetic opioid) in urine samples. The porous surface structure of the sol-gel/Cu nanocomposite coating was revealed by scanning electron microscopy. Direct immersion SPME followed by HPLC-UV determination was employed. The factors influencing the SPME procedure, such as the salt content, desorption solvent type, pH and equilibration time, were optimized. The best conditions were obtained with no salt content, acetonitrile as desorption solvent type, pH 9 and 10 min equilibration time. The calibration graphs for urine samples showed good linearity. The detection limit was about 0.2 ng mL . Also, the novel method for preparation of nanocomposite fiber was compared with previously reported techniques for MDN determination. The results show that the novel nanocomposite fiber has relatively high extraction efficiency.
电化学共沉积溶胶-凝胶/Cu纳米复合材料已被引入作为一种新颖、简单的单步技术，用于制备固相微萃取（SPME）涂层，以萃取尿液样本中的美沙酮（MDN，一种合成阿片类药物）。通过扫描电子显微镜揭示了溶胶-凝胶/Cu纳米复合涂层的多孔表面结构。采用直接浸入式SPME随后进行HPLC-UV测定。对影响SPME过程的因素，如盐含量、解吸溶剂类型、pH值和平衡时间进行了优化。在无盐含量、乙腈作为解吸溶剂类型、pH值为9且平衡时间为10分钟的条件下获得了最佳结果。尿液样本的校准曲线显示出良好的线性。检测限约为0.2 ng/mL。此外，将制备纳米复合纤维的新方法与先前报道的MDN测定技术进行了比较。结果表明，新型纳米复合纤维具有相对较高的萃取效率。
