Beijing National Laboratory for Molecular Sciences; Key Lab of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, People's Republic of China.
University of Chinese Academy of Sciences , Beijing 100049, People's Republic of China.
Anal Chem. 2017 Feb 7;89(3):2080-2085. doi: 10.1021/acs.analchem.6b04722. Epub 2017 Jan 11.
A microfluidic droplet synthesis approach for the preparation of poly N-isopropylacrylamide protected gold nanoparticles (PNIPAm@AuNPs) was presented here. Well-dispersed PNIPAm@AuNPs could be generated within 8 min. On the basis of the aggregation-induced UV-vis adsorption intensity increasing mechanism, the PNIPAm@AuNPs-based colorimetric probe displayed high sensitivity and good selectivity for sensing copper ions. A linear calibration of relative UV-vis adsorption intensity increasing versus copper ions concentration was obtained within 5.0-750.0 μM, and the limit of detection was 2.5 μM. Furthermore, after copper ions were injected in rat, a metabolic assay was developed with the proposed probe. The results indicated that the droplet microfluidic synthesis system could provide a new way for preparation of polymer@AuNPs with good polydispersity index and showed great potential of polymer@AuNPs-based sensing probe for application in biological and clinical analysis.
本文提出了一种微流控液滴合成方法，用于制备聚 N-异丙基丙烯酰胺保护的金纳米粒子（PNIPAm@AuNPs）。在 8 分钟内可以生成分散良好的 PNIPAm@AuNPs。基于聚集诱导的紫外-可见吸收强度增加机制，基于 PNIPAm@AuNPs 的比色探针对铜离子具有高灵敏度和良好的选择性。在 5.0-750.0 μM 范围内，相对紫外-可见吸收强度增加与铜离子浓度之间存在线性校准，检测限为 2.5 μM。此外，在向大鼠中注射铜离子后，使用该探针开发了代谢测定法。结果表明，液滴微流控合成系统可为具有良好多分散指数的聚合物@AuNPs 的制备提供一种新方法，并且基于聚合物@AuNPs 的传感探针在生物和临床分析中的应用具有很大的潜力。
