Qin Xianming, Wang Hairong, Wei Xueyong
State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University Xi'an 710049 China
RSC Adv. 2020 Mar 20;10(20):11565-11572. doi: 10.1039/d0ra01512a. eCollection 2020 Mar 19.
Particle enrichment is an important preparation or collection process in biomedical and biochemical experiments, but the enrichment process in droplets is harder to realize than in continuous fluid. Here we demonstrate an on-chip, label-free and controllable intra-droplet particle enrichment realized in a focused acoustic field. In this process, droplets containing microparticles are trapped, merged together and split off in the focused acoustic region, resulting in droplets with particles of high concentration. By changing the experimental conditions, the degree of enrichment of this method can be tuned up to 26 times, which enables it to meet requirements for sample preparation in various applications.
颗粒富集是生物医学和生化实验中的一个重要制备或收集过程，但与连续流体相比，液滴中的富集过程更难实现。在此，我们展示了一种在聚焦声场中实现的片上、无标记且可控的液滴内颗粒富集。在这个过程中，含有微粒的液滴在聚焦声区域被捕获、合并在一起并分离，从而得到具有高浓度颗粒的液滴。通过改变实验条件，该方法的富集程度可调节至26倍，这使其能够满足各种应用中样品制备的要求。
