Suzuki Atsuko, Saeki Toshiyuki, Ikuji Hiroko, Uchida Chiyoko, Uchida Takafumi
Molecular Enzymology, Department of Molecular Cell Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Amamiya, Tsutsumidori, Aoba, Sendai, Miyagi, Japan.
Department of Human Development and Culture, Fukushima University, Kanayagawa 1, Fukushima, Fukushima, Japan.
PLoS One. 2016 Dec 30;11(12):e0168830. doi: 10.1371/journal.pone.0168830. eCollection 2016.
While screening for an inhibitor of the peptidyl prolyl cis/trans isomerase, Pin1, we came across a brown algae polyphenol that blocks the differentiation of fibroblasts into adipocytes. However, its effectiveness on the accumulation of fat in the body has never been studied.
METHODOLOGY/PRINCIPAL FINDINGS: Oral administration of brown algae polyphenol to mice fed with a high fat diet, suppressed the increase in fat volume to a level observed in mice fed with a normal diet. We speculate that Pin1 might be required for the differentiation of stem cell to adipocytes. We established wild type (WT) and Pin1-/- (Pin1-KO) adipose-derived mesenchymal stem cell (ASC) lines and found that WT ASCs differentiate to adipocytes but Pin1-KO ASCs do not.
Oral administration of brown algae polyphenol, a Pin1 inhibitor, reduced fat buildup in mice. We showed that Pin1 is required for the differentiation of stem cells into adipocytes. We propose that oral intake of brown algae polyphenol is useful for the treatment of obesity.
在筛选肽基脯氨酰顺/反异构酶Pin1的抑制剂时，我们发现了一种褐藻多酚，它能阻止成纤维细胞分化为脂肪细胞。然而，其对体内脂肪积累的作用从未被研究过。
方法/主要发现：给高脂饮食喂养的小鼠口服褐藻多酚，可将脂肪量的增加抑制到正常饮食喂养小鼠所观察到的水平。我们推测Pin1可能是干细胞分化为脂肪细胞所必需的。我们建立了野生型（WT）和Pin1基因敲除（Pin1-KO）脂肪来源间充质干细胞（ASC）系，发现WT ASC可分化为脂肪细胞，而Pin1-KO ASC则不能。
口服Pin1抑制剂褐藻多酚可减少小鼠体内脂肪堆积。我们表明Pin1是干细胞分化为脂肪细胞所必需的。我们提出口服褐藻多酚对治疗肥胖症有用。
