Molecular Enzymology, Department of Molecular Cell Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Amamiya, Tsutsumidori, Aoba, Sendai, Miyagi 981-8555, Japan.
Biochem Biophys Res Commun. 2012 Oct 5;426(4):468-74. doi: 10.1016/j.bbrc.2012.08.094. Epub 2012 Sep 6.
Mesenchymal stem cells (MSCs) can differentiate into a variety of cell types. MSCs exist in several tissues such as the bone marrow, adipose, muscle, cartilage, and tendon. This differentiation potential makes MSCs candidates for cell-based therapeutic strategies for mesenchymal tissue injuries. MSCs can be prepared from bone marrow (BM-MSCs) and adipose (AD-MSCs); however, these MSCs exhibit senescence-associated growth arrest and display inevitable heterogeneity. We established several AD-MSC cell lines from a p53-knockout (KO) mouse. These cell lines were immortalized, but no cell lines grew anchorage-independently, suggesting that they are not cancerous. They differentiated into adipocytes, osteoblasts, and chondrocytes by treatment with certain stimuli. Moreover, following injection into the tail vein, the cells migrated into the wounded region of the liver and differentiated into hepatocytes. We succeeded in establishing several AD-MSC clonal cell lines that maintain the tissue-specific markers and characteristics of the developmental phase. These clonal cell lines will serve as important tools to study the mechanism of differentiation of MSCs.
间充质干细胞（MSCs）可以分化为多种细胞类型。MSCs 存在于几种组织中，如骨髓、脂肪、肌肉、软骨和肌腱。这种分化潜能使 MSCs 成为基于细胞的间充质组织损伤治疗策略的候选者。MSCs 可以从骨髓（BM-MSCs）和脂肪（AD-MSCs）中制备；然而，这些 MSCs 表现出与衰老相关的生长停滞，并表现出不可避免的异质性。我们从 p53 敲除（KO）小鼠中建立了几个 AD-MSC 细胞系。这些细胞系被永生化，但没有细胞系能在无锚定的情况下生长，这表明它们不是癌性的。它们通过用特定的刺激物处理而分化为脂肪细胞、成骨细胞和软骨细胞。此外，将细胞注入尾静脉后，细胞迁移到肝脏受损区域并分化为肝细胞。我们成功地建立了几个保持组织特异性标记物和发育阶段特征的 AD-MSC 克隆细胞系。这些克隆细胞系将成为研究 MSCs 分化机制的重要工具。
