Teti A, Grano M, Teitelbaum S L, Hruska K A, Colucci S, Zambonin Zallone A
Boll Soc Ital Biol Sper. 1989 Aug;65(8):689-93.
In this study the effects of changes in intracellular pH on cytosolic Ca2+ were examined in single isolated osteoclasts. Alkalinization, performed by incubation in HCO3 containing buffer, induced increases in [Ca2+]. Conversely acidification, obtained by incubation in Na-butyrate-containing buffer induced a rapid and sustained decrease of [Ca2+]. The decrease of [Ca2+], during acidification with Na-butyrate was inhibited by VO4, an inhibitor of the Ca2+-ATPase. All these pieces of information indicate that changes in pH1 can modulate osteoclast activity together with modifications of [Ca2+], probably acting as intracellular signals.
在本研究中，我们在单个分离的破骨细胞中检测了细胞内pH变化对胞质Ca2+的影响。通过在含HCO3的缓冲液中孵育进行碱化处理，可诱导[Ca2+]升高。相反，通过在含丁酸钠的缓冲液中孵育实现酸化处理，则会导致[Ca2+]迅速且持续下降。在用丁酸钠酸化过程中，[Ca2+]的下降受到Ca2+-ATP酶抑制剂VO4的抑制。所有这些信息表明，pH1的变化可能作为细胞内信号，与[Ca2+]的改变一起调节破骨细胞活性。
