Teti A, Grano M, Teitelbaum S L, Hruska K A, Colucci S, Zambonin Zallone A
Boll Soc Ital Biol Sper. 1989 Jul;65(7):597-601.
The effects of changes in intracellular pH (pH1) on the organization of the clear zone of isolated avian osteoclasts in culture were studied. The distribution of podosomes, the close contact areas that mediate the adhesion of osteoclasts to the substrate, was investigated by decoration of microfilaments with fluorescent phalloidin. Intracellular acidification by butyric acid induces significant increase of podosome formation at the level of the clear zone compared to controls. Conversely, alkalinization by HCO3- reduces the percentage of osteoclasts with podosomes. A role of pH1 on the adhesion of the osteoclasts to the substrate is hypothesized.
研究了细胞内pH值（pH1）变化对培养的分离禽类破骨细胞透明区组织的影响。通过用荧光鬼笔环肽标记微丝，研究了介导破骨细胞与底物粘附的紧密接触区域——足体的分布。与对照组相比，丁酸引起的细胞内酸化导致透明区水平的足体形成显著增加。相反，HCO3-引起的碱化降低了有足体的破骨细胞百分比。推测pH1在破骨细胞与底物的粘附中起作用。
