Faculty of Science & Arts, Chemistry Department, 17100 Canakkale, Turkey; Nanoscience and Technology Research and Application Center (NANORAC), Canakkale Onsekiz Mart University, Terzioglu Campus, 17100 Canakkale, Turkey.
Faculty of Science & Arts, Chemistry Department, 17100 Canakkale, Turkey.
Carbohydr Polym. 2017 Mar 1;159:29-38. doi: 10.1016/j.carbpol.2016.12.024. Epub 2016 Dec 9.
Poly(hyaluronic acid) (p(HA)) particles with sizes from few hundred nm to few tens of micrometer were synthesized by using epoxy groups containing crosslinker glycerol diglycidyl ether (GDE) with high yield, 94±5%. P(HA) particles were oxidized by treatment with sodium periodate and then reacted with cationic polyethyleneimine (PEI) at 1:0.5, 1:1, and 1:2 wt ratio of p(HA):PEI to obtain p(HA)-PEI particles. From zeta potential measurements, isoelectronic points of bare p(HA) particles increased to pH 8.7 from 2.7 after modification with cationic PEI. New properties, such as antibacterial property, were attained for p(HA)-PEI after modification. The highest minimum bactericidal concentration (MBC) values were 0.5, 1, and 0.5mg/mL against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis species for 1:0.5 ratio of p(HA)-PEI at 72h incubation time. Moreover, the p(HA)-PEI particles were found to be biocompatible with L929 fibroblast cells, and interestingly, p(HA)-PEI particles were found to inhibit MDA-MB-231 breast and H1299 cancer cell growth depending on amount of PEI in p(HA)-PEI particles.
聚（透明质酸）（p（HA））颗粒的尺寸从几百纳米到几十微米，通过使用含有环氧基团的交联剂甘油二缩水甘油醚（GDE）以高产率 94±5%合成。通过用高碘酸钠处理将 p(HA)颗粒氧化，然后以 p(HA)：PEI 的重量比为 1:0.5、1:1 和 1:2 与阳离子聚乙烯亚胺（PEI）反应，得到 p(HA)-PEI 颗粒。从zeta 电位测量可知，未经修饰的 p(HA)颗粒的等电点从 2.7 增加到修饰后的 pH 8.7。修饰后，p(HA)-PEI 获得了新的特性，如抗菌性能。在 72 小时孵育时间下，p(HA)-PEI 对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌的最低杀菌浓度（MBC）值最高分别为 0.5、1 和 0.5mg/mL，其 p(HA)-PEI 与 1:0.5 的比例。此外，p(HA)-PEI 颗粒被发现与 L929 成纤维细胞具有生物相容性，有趣的是，p(HA)-PEI 颗粒被发现抑制 MDA-MB-231 乳腺癌和 H1299 癌细胞的生长，这取决于 p(HA)-PEI 颗粒中 PEI 的含量。
