Jin Xiaoxia, Gong Pengtao, Li Guojiang, Zhang Xichen, Li Jianhua
Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xian Road, Changchun, 130062, China.
Jilin Agricultural Science and Technology University, 77 Hanlin Road, Jilin, 132101, China.
Parasitol Res. 2017 Feb;116(2):813-819. doi: 10.1007/s00436-016-5346-1. Epub 2016 Dec 30.
The Apicomplexan parasite Neospora caninum is an obligate intracellular parasitic protozoan. It can cause severe diseases in a number of animals throughout the world. Infection with N. caninum leads to abortions in pregnant animals and neuromuscular disorders of newborns which cause great economic losses to animal husbandry. However, the mechanism of cell invasion by N. caninum is still unclear. This paper aims to investigate the impact of SB203580, a p38 MAPK inhibitor, on host cell invasion by N. caninum. The results suggested the presence of putative p38 MAPK homologues in N. caninum, and incubation of N. caninum with SB203580 markedly reduced the tachyzoite motility and microneme exocytosis (NcMIC2, 3, and 6). Furthermore, treatment or pretreatment of MDBK cells with SB203580 effectively reduced cell invasion by N. caninum. Therefore, SB203580 affected both, parasites and host cells, resulting in inhibition of cell invasion by N. caninum.
顶复门寄生虫犬新孢子虫是一种专性细胞内寄生原生动物。它可在世界各地的多种动物中引发严重疾病。犬新孢子虫感染会导致怀孕动物流产以及新生动物出现神经肌肉紊乱，给畜牧业造成巨大经济损失。然而，犬新孢子虫入侵细胞的机制仍不清楚。本文旨在研究p38丝裂原活化蛋白激酶（MAPK）抑制剂SB203580对犬新孢子虫入侵宿主细胞的影响。结果表明犬新孢子虫中存在假定的p38 MAPK同源物，用SB203580孵育犬新孢子虫可显著降低速殖子的运动性和微线体胞吐作用（NcMIC2、3和6）。此外，用SB203580处理或预处理MDBK细胞可有效降低犬新孢子虫对细胞的入侵。因此，SB203580对寄生虫和宿主细胞均有影响，从而抑制了犬新孢子虫对细胞的入侵。
