非锚定细胞壁蛋白NCW-1的破坏通过增加粗糙脉孢菌中纤维二糖的摄取来促进纤维素酶的产生。
Disruption of non-anchored cell wall protein NCW-1 promotes cellulase production by increasing cellobiose uptake in Neurospora crassa.
作者信息
Lin Liangcai, Chen Yong, Li Jingen, Wang Shanshan, Sun Wenliang, Tian Chaoguang
机构信息
Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
出版信息
Biotechnol Lett. 2017 Apr;39(4):545-551. doi: 10.1007/s10529-016-2274-1. Epub 2016 Dec 30.
OBJECTIVES
To elucidate the mechanism of cellulase signal transduction in filamentous fungi including the components of the cellulase induction pathway.
RESULTS
Neurospora crassa ncw-1 encodes a non-anchored cell wall protein. The absence of ncw-1 increased cellulase gene expression and this is not due to relieving carbon catabolite repression mediated by the cre-1 pathway. A mutant lacking genes encoding both three major β-glucosidase enzymes and NCW-1 (Δ3βGΔncw-1) was constructed. Transcriptome analysis of the quadruple mutant demonstrated enhanced expression of cellodextrin transporters after ncw-1 deletion, indicating that ncw-1 affects cellulase expression and production by inhibiting the uptake of the cellodextrin.
CONCLUSIONS
NCW-1 is a novel component that plays a critical role in the cellulase induction signaling pathway.
目的
阐明丝状真菌中纤维素酶信号转导的机制，包括纤维素酶诱导途径的组成成分。
结果
粗糙脉孢菌的ncw-1编码一种非锚定细胞壁蛋白。ncw-1缺失会增加纤维素酶基因的表达，这并非是由于解除了由cre-1途径介导的碳代谢物阻遏作用。构建了一个同时缺失编码三种主要β-葡萄糖苷酶和NCW-1的基因的突变体（Δ3βGΔncw-1）。对该四重突变体的转录组分析表明，ncw-1缺失后纤维糊精转运蛋白的表达增强，这表明ncw-1通过抑制纤维糊精的摄取来影响纤维素酶的表达和产生。
结论
NCW-1是纤维素酶诱导信号通路中发挥关键作用的一种新成分。
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