Ozcan Gul, Dagdeviren Ozsoylemez Ozlem, Akman Gizem, Khalilia Walid, Tezel Yetiz Beliz, Karagoz Ali, Melikoglu Gulay, Anil Sezin, Kultur Sukran, Sutlupinar Nurhayat
Department of Biology, Istanbul University, Faculty of Science, Istanbul, Turkey.
J BUON. 2016 Nov-Dec;21(6):1552-1560.
Cancer is a long process that leads the organism to death and is associated with the normal cells acquiring the ability to divide permanently. Nowadays, the use of natural products in cancer therapy has a great importance. In addition, working with plants that are endemic to Turkey and determining the biological activities of these plant extracts, is extremely important due to the potential for new drug development. There is no comparative study available in the literature on the antitumor effects of Colchicum sanguicolle, a new found species of the genus Colchicum in Turkey, Crateagus microphylla, of the genus Crateagus and Centaurea antiochia of the genus Centaurea. In this study, we tried to demonstrate the antitumor effect of these plant extracts on HeLa and C 4-1 cells.
Five different doses (0.001, 0.01, 0.05, 0.25 and 0.5 mg/ml) of the three plant types were prepared and applied for 24, 48 and 72 hrs on the cervical cancer derived cell lines. Subsequently, the growth rate was evaluated with the mitochondrial dehydrogenase enzyme method.
Colchicum sanguicolle extracts showed the most effective antitumor activity. For the Colchicum sanguicolle extract, the IC50 dose for HeLa cells was 0.01 mg/ml at 48 hrs, while for the C-4 I cells it was 0.001 mg/ml at 48 hrs. These results showed that C-4 I cells were more sensitive to the Colchicum sanguicolle extracts. Conclus?on: The results of from this study regarding the antitumor effect of plant extracts of endemic varieties of Turkey may have an important place in design and development of anticancer drugs and would make contributions to other studies to be conducted in this area.
癌症是一个导致机体死亡的漫长过程，与正常细胞获得永久分裂能力有关。如今，天然产物在癌症治疗中的应用具有重要意义。此外，研究土耳其特有的植物并确定这些植物提取物的生物活性，对于新药开发潜力而言极为重要。文献中尚无关于土耳其新发现的秋水仙属物种血红秋水仙、山楂属的小叶山楂和矢车菊属的安条克矢车菊抗肿瘤作用的比较研究。在本研究中，我们试图证明这些植物提取物对HeLa和C 4 - 1细胞的抗肿瘤作用。
制备三种植物的五种不同剂量（0.001、0.01、0.05、0.25和0.5 mg/ml），并将其应用于宫颈癌衍生细胞系24、48和72小时。随后，采用线粒体脱氢酶法评估生长速率。
血红秋水仙提取物显示出最有效的抗肿瘤活性。对于血红秋水仙提取物，HeLa细胞在48小时时的IC50剂量为0.01 mg/ml，而C - 4 I细胞在48小时时为0.001 mg/ml。这些结果表明C - 4 I细胞对血红秋水仙提取物更敏感。结论：本研究关于土耳其特有品种植物提取物抗肿瘤作用的结果，可能在抗癌药物的设计和开发中占有重要地位，并将为该领域的其他研究做出贡献。
