余甘子种子提取物介导的 PdNPs 的合成及其对抗菌、溶血和细胞毒性的研究。
Phyllanthus emblica seed extract mediated synthesis of PdNPs against antibacterial, heamolytic and cytotoxic studies.
机构信息
Department of Biotechnology, School of Biosciences and Technology, VIT University, Vellore 632 014, Tamilnadu, India.
Chemistry of Heterocycles & Natural Product Research Laboratory, Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632 014, Tamilnadu, India.
出版信息
J Photochem Photobiol B. 2017 Feb;167:64-71. doi: 10.1016/j.jphotobiol.2016.12.012. Epub 2016 Dec 22.
Ecofriendly synthesis of Palladium nanoparticles (PdNPs) were achieved using Phyllanthus emblica (P. emblica) seeds as reducing agent. Further the ecofriendly synthesized PdNPs were subjected for various analytical techniques like UV-Vis, FT-IR, XRD, Zeta potential, SEM and TEM. The results indicated that green synthesized PdNPs were spherical in shape with average particle size of 28±2nm with moderate stability. Further the synthesized PdNPs and extract were subjected for its antibacterial studies against various disease causing pathogens by agar well diffusion method. Seed extract resulted in 8.9±1.46mm against B. subtilis and PdNPs showed 9.6±1.10mm against S. aureus and synthesized PdNPs and extract were tested for hemolytic which resulted in 20% and 10% respectively. Toxicity studies were done against Artemia salina (A. salina). The LC value of green synthesized P. emblica capped PdNPs and the P. emblica seed extract were found to be less toxic for A. salina with a value of 1.00μg/mL and 1.25μg/mL. In addition samples were checked for in vitro cytotoxicity assays on HeLa cell lines.
采用余甘子（P. emblica）种子作为还原剂，实现了钯纳米粒子（PdNPs）的环保合成。进一步地，将环保合成的 PdNPs 进行了各种分析技术的测试，如 UV-Vis、FT-IR、XRD、Zeta 电位、SEM 和 TEM。结果表明，绿色合成的 PdNPs 呈球形，平均粒径为 28±2nm，具有中等稳定性。进一步地，将合成的 PdNPs 和提取物进行琼脂孔扩散法抗菌研究，以评估其对各种致病菌的抑制效果。种子提取物对枯草芽孢杆菌的抑菌圈直径为 8.9±1.46mm，而 PdNPs 对金黄色葡萄球菌的抑菌圈直径为 9.6±1.10mm。此外，还对合成的 PdNPs 和提取物进行了溶血试验，结果分别为 20%和 10%。对卤虫（Artemia salina，A. salina）进行了毒性研究。绿色合成的余甘子封端 PdNPs 和余甘子种子提取物对 A. salina 的 LC 值分别为 1.00μg/mL 和 1.25μg/mL，表明它们的毒性较低。此外，还对 HeLa 细胞系进行了体外细胞毒性试验，以评估样品的安全性。
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