İlhan Mert, Süntar İpek, Demirel Mürşide Ayşe, Yeşilada Erdem, Keleş Hikmet, Küpeli Akkol Esra
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler, Ankara, Turkey.
Laboratory Animals Breeding and Experimental Researches Center, Faculty of Pharmacy, Gazi University, Etiler, Ankara, Turkey.
Taiwan J Obstet Gynecol. 2016 Dec;55(6):786-790. doi: 10.1016/j.tjog.2015.01.006.
Sea buckthorn (Hippophae rhamnoides L.) and St. John's wort (Hypericum perforatum L.) are used as an emmenagog and for the treatment of other gynecological disorders including uterus inflammation and endometriosis. The aim of the present study is to investigate the potential of a mixture of sea buckthorn and St. John's wort oils (HrHp oil) in the treatment of endometriosis.
The activity was assessed in surgically induced endometriosis in rats. A 15-mm piece of endometrium was sutured into the abdominal wall. Twenty-eight days later, a second laparotomy was performed to calculate the endometrial foci areas and to score intra-abdominal adhesions. The rats were treated with either vehicle, HrHp oil formulation, or the reference (buserelin acetate). At the end of the experiment all rats were sacrificed and endometriotic foci areas and intra-abdominal adhesions were re-evaluated. The tissue sections were analyzed histopathologically. Peritoneal fluids of the experimental animals were collected in order to detect the levels of tumor necrosis factor-α, vascular endothelial growth factor, and interleukin-6, which might be involved in the etiology of endometriosis.
In the HrHp oil-treated group, the volumes of endometriotic implants were found to be significantly decreased (from 50.8 mm to 18.6 mm, p<0.001) without any adhesion (0.0±0.0, p<0.001) when compared to the control group (3.1±0.9). The levels of tumor necrosis factor-α decreased from 7.02±1.33 pg/mL to 4.78±1.02 pg/mL (p<0.01); vascular endothelial growth factor from 17.39±8.52 pg/mL to 9.67±5.04 pg/mL (p<0.01); and interleukin-6 from 50.95±22.84 pg/mL to 29.11±7.45 pg/mL (p<0.01), respectively, after HrHp oil treatment.
HrHp oil may be a promising alternative for the treatment of endometriosis.
沙棘（沙棘属）和圣约翰草（贯叶连翘）被用作通经药，并用于治疗包括子宫炎症和子宫内膜异位症在内的其他妇科疾病。本研究的目的是探究沙棘油和圣约翰草油混合物（HrHp油）治疗子宫内膜异位症的潜力。
在手术诱导的大鼠子宫内膜异位症模型中评估其活性。将一块15毫米的子宫内膜缝合到腹壁中。28天后，进行第二次剖腹手术以计算子宫内膜病灶面积并对腹腔内粘连进行评分。用赋形剂、HrHp油制剂或参比物（醋酸布舍瑞林）对大鼠进行治疗。实验结束时，处死所有大鼠，并重新评估子宫内膜异位病灶面积和腹腔内粘连情况。对组织切片进行组织病理学分析。收集实验动物的腹腔液，以检测可能与子宫内膜异位症病因有关的肿瘤坏死因子-α、血管内皮生长因子和白细胞介素-6的水平。
与对照组（3.1±0.9）相比，在HrHp油治疗组中，发现子宫内膜异位植入物的体积显著减小（从50.8立方毫米降至18.6立方毫米，p<0.001），且无任何粘连（0.0±0.0，p<0.001）。HrHp油治疗后，肿瘤坏死因子-α水平从7.02±1.33皮克/毫升降至4.78±1.02皮克/毫升（p<0.01）；血管内皮生长因子从17.39±8.52皮克/毫升降至9.67±5.04皮克/毫升（p<0.01）；白细胞介素-6从50.95±22.84皮克/毫升降至29.11±7.45皮克/毫升（p<0.01）。
HrHp油可能是治疗子宫内膜异位症的一种有前景的替代方法。
