Liver Unit, Liver Disease Laboratory-Viral Hepatitis, Internal Medicine Department, Vall d'Hebron Institut Research-Hospital Universitari Vall d'Hebron (VHIR-HUVH), Universitat Autonoma de Barcelona, 08035, Barcelona, Spain; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Barcelona, Spain; Universitat Autònoma de Barcelona, Barcelona, Spain.
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Barcelona, Spain; Liver Pathology Unit, Departments of Biochemistry and Microbiology, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona (UAB), Barcelona, Spain.
Virus Res. 2017 Jul 15;239:115-125. doi: 10.1016/j.virusres.2016.12.020. Epub 2016 Dec 28.
The hepatitis viruses represent a major public health problem worldwide. Procedures for characterization of the genomic composition of their populations, accurate diagnosis, identification of multiple infections, and information on inhibitor-escape mutants for treatment decisions are needed. Deep sequencing methodologies are extremely useful for these viruses since they replicate as complex and dynamic quasispecies swarms whose complexity and mutant composition are biologically relevant traits. Population complexity is a major challenge for disease prevention and control, but also an opportunity to distinguish among related but phenotypically distinct variants that might anticipate disease progression and treatment outcome. Detailed characterization of mutant spectra should permit choosing better treatment options, given the increasing number of new antiviral inhibitors available. In the present review we briefly summarize our experience on the use of deep sequencing for the management of hepatitis virus infections, particularly for hepatitis B and C viruses, and outline some possible new applications of deep sequencing for these important human pathogens.
肝炎病毒是全球主要的公共卫生问题。需要对其种群的基因组组成进行特征描述、准确诊断、多重感染的鉴定，以及有关治疗决策的抑制剂逃逸突变体的信息。由于这些病毒作为复杂和动态的准种群体进行复制，其复杂性和突变体组成是具有生物学相关性的特征，因此深度测序方法对这些病毒非常有用。种群复杂性是疾病预防和控制的主要挑战，但也是区分相关但表型不同的变体的机会，这些变体可能预示着疾病进展和治疗结果。鉴于可用的新型抗病毒抑制剂数量不断增加，详细描述突变谱应该可以为选择更好的治疗方案提供依据。在本综述中，我们简要总结了我们在使用深度测序管理肝炎病毒感染方面的经验，特别是乙型和丙型肝炎病毒，并概述了深度测序在这些重要人类病原体方面的一些可能的新应用。
