de Carvalho Tiago Pinheiro Vaz, Ferrari Teresa Cristina Abreu, de Santana Josimari Melo, Viana Victor Antônio Santos, Santos José Aislan Correia, do Nascimento Wheverton Correia, da Cruz Kamilla Mayara Lucas, de Araújo Karina Conceição Gomes Machado
Centro de Pesquisas Aggeu Magalhães - FIOCRUZ, Recife, Pernambuco, Brazil.
Universidade Federal de Minas Gerais - UFMG, Belo Horizonte, Minas Gerais, Brazil.
Acta Trop. 2017 Mar;167:142-147. doi: 10.1016/j.actatropica.2016.12.028. Epub 2016 Dec 28.
Schistosomal myeloradiculopathy (SMR) is a severe form of presentation of schistosomiasis in which Schistosoma spp. affect the spinal cord. The aims of the present study were to develop an animal model of SMR caused by S. mansoni, to characterize both sensory and motor abnormalities in the infected animals, and to investigate the relationship of the sensory, motor and histological findings with the progression of the infection over time. Mechanical sensitivity and behavioral tests were performed followed by euthanasia in male Wistar rats divided into six groups of five animals each, on days 5, 10, 20 and 30 after infection of S. mansoni eggs. The controls were subjected to the same procedure but were administered phosphate-buffered saline (PBS). The spinal cord was removed and subjected to histological analysis. S. mansoni eggs were found in the spinal cord of 25% of the infected animals, which belonged to the groups that exhibited more significant reduction of the superficial mechanical sensitivity, thermal sensitivity and muscle strength. This model proved to be satisfactory to assess functional changes in Wistar rats and might be used in studies investigating the pathogenesis of SMR. To our knowledge, this is the first experimental model of SMR.
血吸虫性脊髓神经根病（SMR）是血吸虫病的一种严重表现形式，其中血吸虫属会影响脊髓。本研究的目的是建立由曼氏血吸虫引起的SMR动物模型，对受感染动物的感觉和运动异常进行表征，并研究感觉、运动和组织学结果与感染随时间进展之间的关系。在感染曼氏血吸虫卵后的第5、10、20和30天，对分成六组、每组五只动物的雄性Wistar大鼠进行机械敏感性和行为测试，然后实施安乐死。对照组接受相同程序，但给予磷酸盐缓冲盐水（PBS）。取出脊髓并进行组织学分析。在25%的受感染动物的脊髓中发现了曼氏血吸虫卵，这些动物属于表现出更明显的浅表机械敏感性、热敏感性和肌肉力量降低的组。该模型被证明可令人满意地评估Wistar大鼠的功能变化，并可用于研究SMR发病机制的研究。据我们所知，这是首个SMR实验模型。
