Guderley H, Jean C, Blouin M
Département de biologie, Université Laval, Québec, Canada.
Biochim Biophys Acta. 1989 Oct 26;977(1):87-90. doi: 10.1016/s0005-2728(89)80012-x.
Fatigue of isolated gastrocnemius muscles from R. pipiens leads to a marked increase in the proportion of phosphofructokinase bound to the particulate fraction and a decrease in the binding of lactate dehydrogenase, pyruvate kinase, creatine phosphokinase and glyceraldehyde-3-phosphate dehydrogenase. Only the proportion of aldolase bound to the particulate fraction was unaffected by fatigue. This pattern was unchanged when fatigued muscles were extracted at pH 6.5 rather than 7.5. Thus, muscle fatigue leads to opposite changes in the binding of the glycolytic enzymes.
豹蛙离体腓肠肌疲劳会导致与颗粒部分结合的磷酸果糖激酶比例显著增加，而乳酸脱氢酶、丙酮酸激酶、肌酸磷酸激酶和甘油醛 - 3 - 磷酸脱氢酶的结合减少。只有与颗粒部分结合的醛缩酶比例不受疲劳影响。当在pH 6.5而非7.5条件下提取疲劳肌肉时，这种模式没有改变。因此，肌肉疲劳会导致糖酵解酶结合发生相反的变化。
