用卤代烃处理的大鼠肝脏的体内和体外31P核磁共振波谱分析。

In vivo and in vitro 31P-NMR spectroscopy of rat liver treated with halocarbons.

作者信息

Towner R A, Brauer M, Janzen E G, Ling M F

机构信息

Guelph-Waterloo Centre for Graduate Work in Chemistry, University of Guelph, Canada.

出版信息

Biochim Biophys Acta. 1989 Oct 13;993(1):92-9. doi: 10.1016/0304-4165(89)90147-5.

DOI:10.1016/0304-4165(89)90147-5

PMID:2804127

Abstract

Both in vivo and in vitro 31P-NMR spectroscopy were used to demonstrate metabolic changes in rat liver as a function of time after exposure to either carbon tetrachloride (CCl4) or bromotrichloromethane (BrCCl3). The inorganic phosphate resonance, measured in vivo, moves upfield, which is associated with a decrease in cytosolic pH over a 12 or 20 h period (for BrCCl3 or CCl4, respectively). Intoxication by CCl4 or BrCCl3 causes an intracellular acidosis to pH 7.05 or 6.82 (+/- 0.05), respectively. Also, it has been found that halocarbon exposure increases the amounts of phosphomonoesters (PME) detected. High resolution in vitro 31P-NMR spectroscopy studies of perchloric acid extracts of CCl4-treated rat livers indicated a significant increase in the height of the phosphocholine resonance in the PME region 4-5 h after CCl4 exposure.

摘要

体内和体外³¹P核磁共振波谱法均被用于证明大鼠肝脏在接触四氯化碳（CCl₄）或三氯溴甲烷（BrCCl₃）后随时间变化的代谢改变。体内测量的无机磷酸盐共振峰向高场移动，这与在12小时或20小时内（分别对应BrCCl₃或CCl₄）胞质pH值降低有关。CCl₄或BrCCl₃中毒分别导致细胞内酸中毒至pH 7.05或6.82（±0.05）。此外，还发现接触卤代烃会增加所检测到的磷酸单酯（PME）的量。对CCl₄处理的大鼠肝脏的高氯酸提取物进行的体外³¹P核磁共振高分辨率波谱研究表明，在CCl₄暴露后4 - 5小时，PME区域中磷酸胆碱共振峰的高度显著增加。