胰腺解痉多肽的修订氨基酸序列与在一种人类乳腺癌细胞系中发现的可诱导性pS2肽表现出更高的相似性。

Revised amino acid sequence of pancreatic spasmolytic polypeptide exhibits greater similarity with an inducible pS2 peptide found in a human breast cancer cell line.

作者信息

Rose K, Savoy L A, Thim L, Christensen M, Jørgensen K H

机构信息

Département de Biochimie Médicale, C.M.U. Geneva, Switzerland.

出版信息

Biochim Biophys Acta. 1989 Oct 19;998(3):297-300. doi: 10.1016/0167-4838(89)90288-4.

DOI:10.1016/0167-4838(89)90288-4

PMID:2804132

Abstract

The published amino acid sequence of pancreatic spasmolytic polypeptide (Thim, L., Thomsen, J., Christensen, M. and Jørgensen, K.H. et al. (1985) Biochim. Biophys. Acta 827, 410-418) has been checked by a combination of mass spectroscopy and Edman degradation. The pyroglutamyl blocking group was positively identified, and residue assignments at four positions were corrected: Lys48 (not Ser), Ser63 (not Lys), Cys68 (not Ser) and Ser74 (not Cys). The revised sequence exhibits greater similarity with pS2 peptide, a 60 residue polypeptide which is induced by oestrogen in the human breast cancer cell line MCF-7 and found in malignant but not in non-malignant breast tissue.

摘要

胰腺解痉多肽已发表的氨基酸序列（蒂姆，L.，汤姆森，J.，克里斯蒂安森，M.和乔根森，K.H.等人（1985年），《生物化学与生物物理学学报》827卷，410 - 418页）已通过质谱和埃德曼降解相结合的方法进行了核对。焦谷氨酰封闭基团得到了明确鉴定，四个位置的残基归属得到了校正：赖氨酸48（不是丝氨酸）、丝氨酸63（不是赖氨酸）、半胱氨酸68（不是丝氨酸）和丝氨酸74（不是半胱氨酸）。修订后的序列与pS2肽有更高的相似性，pS2肽是一种由雌激素在人乳腺癌细胞系MCF - 7中诱导产生、在恶性乳腺组织而非良性乳腺组织中发现的60个残基的多肽。