[光谱紫外真空区域中总组蛋白和pBr - 322质粒DNA的光解作用]
[Photolysis of total histone and pBr-322 plasmid DNA in the ultraviolet vacuum region of the spectrum].
作者信息
Kiseleva M N, Tsyganenko N M, Smirnova T M, Dodonova N Ia
出版信息
Biofizika. 1989 Jul-Aug;34(4):536-40.
The action of VUV and far UV (lambda greater than 240 nm) radiation of aqueous solutions of total histone by means of electrophoresis method is compared. By VUV photolysis the more effective destruction of HI and HIA histone fractions is determined. It may be explained by interaction of total histone with water radicals (H, OH) formed by VUV photodissociation (indirect mechanism). By VUV irradiation of plasmid pBR-322 DNA in aqueous solutions the indirect action mechanism of single strand breaks in DNA is established.
通过电泳法比较了真空紫外（VUV）和远紫外（波长大于240nm）辐射对总组蛋白水溶液的作用。通过VUV光解确定了HI和HIA组蛋白组分更有效的破坏。这可能是由于总组蛋白与VUV光解离形成的水自由基（H、OH）相互作用（间接机制）所致。通过对水溶液中的质粒pBR - 322 DNA进行VUV照射，确定了DNA中单链断裂的间接作用机制。
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