Burchuladze T G, Sideris E G, Fraĭkin G Ia
Biofizika. 1990 Sep-Oct;35(5):722-5.
It has been shown that NADH photosensitize in vitro single-strand breaks formation in double-strand plasmid DNA pBR 322 upon near-UV (320-400 nm) irradiation. The number of single-strand breaks depends both on UV light dose and sensitizer concentration. Addition of catalase and sodium benzoate strongly decreases the single-strand breaks formation. The results show an important role of hydrogen peroxide (H2O2) and hydroxyl radical (.OH) in inducing single-strand breaks in plasmid DNA irradiated by near-UV radiation in the presence of NADH.
研究表明，在近紫外光（320 - 400纳米）照射下，烟酰胺腺嘌呤二核苷酸（NADH）可在体外使双链质粒DNA pBR 322形成单链断裂。单链断裂的数量取决于紫外光剂量和敏化剂浓度。添加过氧化氢酶和苯甲酸钠可显著减少单链断裂的形成。结果表明，在NADH存在的情况下，过氧化氢（H2O2）和羟基自由基（·OH）在近紫外辐射诱导质粒DNA单链断裂中起重要作用。
