Villard Christina, Eriksson Per, Kronqvist Malin, Lengquist Mariette, Jorns Carl, Hartman Johan, Roy Joy, Hultgren Rebecka
Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden; Department of Vascular Surgery, Karolinska University Hospital, Stockholm, Sweden.
Atherosclerosis Research Unit, Center for Molecular Medicine, Department of Medicine, Karolinska Institutet, Stockholm, Sweden.
Maturitas. 2017 Feb;96:39-44. doi: 10.1016/j.maturitas.2016.11.005. Epub 2016 Nov 11.
Male sex is a significant risk factor for abdominal aortic aneurysm (AAA). Female sex hormones have been reported to prevent aneurysm formation in animal models. The study aims to describe the expression profile of sex hormone receptors in the aneurysm wall of men and women with AAA and compare with unaffected controls.
Aneurysm wall biopsies were obtained during elective open repair of AAA in men and women (n=16+16). Aortic vessel wall from controls were obtained at organ donation (n=6). Western blot-, mRNA expression- and immunohistochemical analyses were performed to assess the expression profile of the sex hormone receptors - androgen receptor (AR), progesterone receptor (PR), estrogen receptor α (ERα) and β (ERβ).
The mRNA- and protein expression levels of AR were higher in AAA compared with control aorta (7.26 vs. 5.14, P=0.001). mRNA- and protein expression levels of ERβ were lower in AAA compared with control aorta (9.15 vs. 12.29, P<0.001). mRNA expression levels of PR were higher in AAA compared with control aorta (8.73 vs. 6.21, P=0.003), but could not be confirmed on protein level. The expression profile of sex hormone receptors in men and women with AAA was similar.
Expression of sex hormone receptors differs in the aneurysmal aorta compared with unaffected aorta in men and women. A higher expression of AR and a lower expression of ERβ suggest that sex hormone activity could be associated with aneurysm development.
男性是腹主动脉瘤（AAA）的重要危险因素。据报道，女性性激素可在动物模型中预防动脉瘤形成。本研究旨在描述AAA男性和女性动脉瘤壁中性激素受体的表达谱，并与未受影响的对照组进行比较。
在男性和女性（n=16+16）择期开放性AAA修复术中获取动脉瘤壁活检组织。在器官捐献时获取对照组的主动脉血管壁（n=6）。进行蛋白质印迹、mRNA表达和免疫组织化学分析，以评估性激素受体——雄激素受体（AR）、孕激素受体（PR）、雌激素受体α（ERα）和β（ERβ）的表达谱。
与对照主动脉相比，AAA中AR的mRNA和蛋白质表达水平更高（7.26对5.14，P=0.001）。与对照主动脉相比，AAA中ERβ的mRNA和蛋白质表达水平更低（9.15对12.29，P<0.001）。与对照主动脉相比，AAA中PR的mRNA表达水平更高（8.73对6.21，P=0.003），但在蛋白质水平上未得到证实。AAA男性和女性中性激素受体的表达谱相似。
与未受影响的男性和女性主动脉相比，动脉瘤性主动脉中性激素受体的表达存在差异。AR表达升高和ERβ表达降低表明性激素活性可能与动脉瘤发展有关。
