Quast Anja, Martian Viktor, Bohnsack Annegret, Batschkus Sarah, Meyer-Marcotty Philipp, Miosge Nicolai
Department of Orthodontics, University Medical Center, Goettingen, Germany.
Tissue Regeneration Group, Department of Orthodontics, University Medical Center, Goettingen, Germany.
Arch Oral Biol. 2021 Feb;122:105026. doi: 10.1016/j.archoralbio.2020.105026. Epub 2020 Dec 9.
This study evaluated the gene expression and protein synthesis of sex hormone receptors in human periodontal ligament cells (PDLCs) in relation to donor- and tooth-specific factors with the aim to clarify the debate about sex hormone receptors in PDLCs.
The expression patterns of estrogen receptors (genes: ESR1 and ESR2; proteins: ERα and ERβ), androgen receptor (AR) and progesterone receptor (PR) were investigated in the context of immortalization status, previous orthodontic tooth movement (OTM), donor age, sex and hormonal stimulation in PDLCs from 14 healthy donors (male: n = 8, female: n = 6; adolescents: n = 8, adults: n = 6) using quantitative real-time polymerase chain reaction, Western blot and immunocytochemistry.
For ERβ, the full-length isoform ERβ1 and truncated variants were detected. For ERα, the expected isoform ERα66 was not observed, but a novel isoform ERα36 was detected. Immortalization status, previous OTM and donor age had no impact on ESR1 and ESR2 expression. Estradiol stimulation for 24 h doubled the ratio of ESR2/ESR1 in PDLCs from female but not male donors, indicating sex-specific patterns of receptor expression. AR and PR demonstrated insufficient protein synthesis in PDLCs.
The data revealed a pivotal role for and complex interplay between ERα and ERβ in human PDLCs regardless of variable donor characteristics. Therefore, PDLC biology might be altered in patients of each age group and both sexes due to hormonal changes. This should be kept in mind during periodontic and orthodontic treatment of patients with special hormonal status.
本研究评估了人牙周膜细胞（PDLCs）中性激素受体的基因表达和蛋白质合成，及其与供体和牙齿特异性因素的关系，旨在阐明关于PDLCs中性激素受体的争论。
在14名健康供体（男性：n = 8，女性：n = 6；青少年：n = 8，成年人：n = 6）的PDLCs中，采用定量实时聚合酶链反应、蛋白质免疫印迹和免疫细胞化学方法，研究雌激素受体（基因：ESR1和ESR2；蛋白质：ERα和ERβ）、雄激素受体（AR）和孕激素受体（PR）在永生化状态、既往正畸牙齿移动（OTM）、供体年龄、性别和激素刺激背景下的表达模式。
对于ERβ，检测到全长异构体ERβ1和截短变体。对于ERα，未观察到预期的异构体ERα66，但检测到一种新型异构体ERα36。永生化状态、既往OTM和供体年龄对ESR1和ESR2表达无影响。雌二醇刺激24小时使女性供体而非男性供体的PDLCs中ESR2/ESR1比值翻倍，表明受体表达存在性别特异性模式。AR和PR在PDLCs中显示蛋白质合成不足。
数据揭示了ERα和ERβ在人PDLCs中起关键作用且相互作用复杂，与供体特征的变化无关。因此，由于激素变化，各年龄组和两性患者的PDLC生物学特性可能会改变。在对具有特殊激素状态的患者进行牙周治疗和正畸治疗时应牢记这一点。
