家兔的性别预选：通过DNA和细胞分选分离X和Y精子后的活产仔。

Sex preselection in rabbits: live births from X and Y sperm separated by DNA and cell sorting.

作者信息

Johnson L A, Flook J P, Hawk H W

机构信息

Reproduction Laboratory, Beltsville Agricultural Research Center, U.S. Department of Agriculture, Maryland 20705.

出版信息

Biol Reprod. 1989 Aug;41(2):199-203. doi: 10.1095/biolreprod41.2.199.

DOI:10.1095/biolreprod41.2.199

PMID:2804212

Abstract

Intact, viable X and Y chromosome-bearing sperm populations of the rabbit were separated according to DNA content with a flow cytometer/cell sorter. Reanalysis for DNA of an aliquot from each sorted population showed purities of 86% for X-bearing sperm and 81% for Y-bearing sperm populations. Sorted sperm were surgically inseminated into the uterus of rabbits. From does inseminated with sorted X-bearing sperm, 94% of the offspring born were females. From does inseminated with sorted Y-bearing sperm from the same ejaculates, 81% of the offspring were males. The probability of the phenotypic sex ratios differing from 50:50 were p less than 0.0003 for X-sorted sperm and p less than 0.004 for Y-sorted sperm. Thus, the phenotypic sex ratio at birth was accurately predicted from the flow-cytometrically measured proportion of X- and Y-bearing sperm used for insemination.

摘要

利用流式细胞仪/细胞分选仪，根据DNA含量对兔体内完整、有活力的携带X和Y染色体的精子群体进行了分离。对每个分选群体的一份等分试样进行DNA再分析，结果显示，携带X染色体的精子群体纯度为86%，携带Y染色体的精子群体纯度为81%。将分选后的精子通过手术方式输精到兔子宫内。在接受分选的携带X染色体精子输精的母兔中，出生的后代94%为雌性。在接受来自相同射精样本的分选后的携带Y染色体精子输精的母兔中，81%的后代为雄性。对于分选后的X染色体精子，表型性别比不同于50:50的概率p小于0.0003；对于分选后的Y染色体精子，p小于0.004。因此，根据流式细胞仪测定的用于输精的携带X和Y染色体精子的比例，可以准确预测出生时的表型性别比。