马来西亚树叶提取物对体外培养基中致病性耳霉菌病相关真菌物种的抗真菌作用

Antifungal Effect of Malaysian Leaf Extract on Selected Fungal Species of Pathogenic Otomycosis Species in In Vitro Culture Medium.

作者信息

Saniasiaya Jeyasakthy, Salim Rosdan, Mohamad Irfan, Harun Azian

机构信息

Department of Otorhinolaryngology-Head & Neck Surgery, School of Medical Sciences, Universiti Sains Malaysia Health Campus, 16150 Kota Bharu, Kelantan, Malaysia.

Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia Health Campus, 16150 Kota Bharu, Kelantan, Malaysia.

出版信息

Oman Med J. 2017 Jan;32(1):41-46. doi: 10.5001/omj.2017.08.

DOI:10.5001/omj.2017.08

PMID:28042402

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5187399/

Abstract

OBJECTIVES

or has been used for therapeutic purposes since ancient times with antifungal activity known to be amongst its medicinal properties. We conducted a pilot study to determine the antifungal properties of Malaysian leaf extract on otomycosis species including and

METHODS

This laboratory-controlled prospective study was conducted at the Universiti Sains Malaysia. Extracts of Malaysian leaf was prepared in ethanol and solutions via the Soxhlet extraction method. Sabouraud dextrose agar cultured with the two fungal isolates were inoculated with the five different concentrations of each extract (50 g/mL, 25 g/mL, 12.5 g/mL, 6.25 g/mL, and 3.125 g/mL) using the well-diffusion method. Zone of inhibition was measured followed by minimum inhibitory concentration (MIC).

RESULTS

For a zone of inhibition for alcohol and aqueous extract was seen for all concentrations except 3.125 g/mL. There was no zone of inhibition for both alcohol and aqueous extracts of leaf for . The MIC values of aqueous and alcohol extracts were 5.1 g/mL and 4.4 g/mL for and since no zone of inhibition was obtained for the MIC was not determined.

CONCLUSIONS

The antifungal effect of alcohol extracts of Malaysian leaf is better than the aqueous extract for ( < 0.001). Malaysian has a significant antifungal effect towards

摘要

目的

自古以来，[植物名称]就因其药用特性中的抗真菌活性而被用于治疗目的。我们进行了一项初步研究，以确定马来西亚[植物名称]叶提取物对包括[真菌种类1]和[真菌种类2]在内的耳霉菌种的抗真菌特性。

方法

这项实验室对照的前瞻性研究在马来西亚理科大学进行。通过索氏提取法，用乙醇和溶液制备马来西亚[植物名称]叶提取物。采用打孔扩散法，将两种真菌分离株接种于含有五种不同浓度（50 g/mL、25 g/mL、12.5 g/mL、6.25 g/mL和3.125 g/mL）的每种提取物的沙氏葡萄糖琼脂培养基上。测量抑菌圈，然后测定最低抑菌浓度（MIC）。

结果

对于[真菌种类1]，除3.125 g/mL外，所有浓度的乙醇提取物和水提取物均可见抑菌圈。对于[真菌种类2]，[植物名称]叶的乙醇提取物和水提取物均未出现抑菌圈。[真菌种类1]的水提取物和乙醇提取物的MIC值分别为5.1 g/mL和4.4 g/mL，由于[真菌种类2]未获得抑菌圈，因此未测定MIC。