Department of Microbiology, Kasturba Medical College Mangalore, Manipal Academy of Higher Education, Karnataka, Manipal, 576104, India.
Infect Disord Drug Targets. 2025;25(1):e280624231439. doi: 10.2174/0118715265301138240605071106.
Antimicrobial resistance exhibited by bacteria against the majority of antibiotics has resulted in research on alternative methods of treatment. Aloe vera has a strong tradition as a medical plant with a wide range of therapeutic uses.
The objective of this study is to determine the antibacterial activity of gel and crude ethanol leaf extract of Aloe vera against Staphylococcus aureus and Enterobacterales isolated from wound infections.
It is a cross-sectional study conducted over a period of 7 months. Antibacterial effect of the ethanol leaf extract and gel was determined by the punch well method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the ethanol leaf extract were determined by macro broth dilution technique.
Aloe vera ethanol leaf extract induced a mean zone size of 13.0 ± 6.0 mm and 16.7 ± 8.4 mm, respectively, for S. aureus and Enterobacterales by Punch Well method (p≤0.002). Whereas Aloe vera gel failed to induce any zone of inhibition for all the isolates p<0.001. Mean MIC of Aloe vera leaf extract against 74 S. aureus was 94 ± 41.23 mg/ml and against 73 Enterobacterales, it was 45.6 ± 20 mg/ml p < 0.001. Mean MBC of Aloe vera leaf extract against 74 S.aureus isolates was 188 ± 82.46 mg/ml and against 73 Enterobacterales was 91.18±40 mg/ml p < 0.001.
Aloe vera ethanol leaf extract showed a good antibacterial effect against the different strains of bacteria causing wound infection. The present article shows the possibility of future use of natural products for the treatment of wound infections.
细菌对抗生素的耐药性导致了对替代治疗方法的研究。 芦荟作为一种具有广泛治疗用途的药用植物具有悠久的传统。
本研究旨在确定芦荟凝胶和粗乙醇叶提取物对金黄色葡萄球菌和肠杆菌科的抗菌活性，这些细菌从伤口感染中分离出来。
这是一项为期 7 个月的横断面研究。 采用打孔法测定乙醇叶提取物和凝胶的抑菌作用。 采用宏肉汤稀释技术测定乙醇叶提取物的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。
芦荟乙醇叶提取物通过打孔法分别诱导金黄色葡萄球菌和肠杆菌科的平均抑菌环直径为 13.0±6.0mm 和 16.7±8.4mm（p≤0.002）。 然而，芦荟凝胶未能诱导所有分离株的任何抑菌环，p<0.001。 芦荟叶提取物对 74 株金黄色葡萄球菌的平均 MIC 为 94±41.23mg/ml，对 73 株肠杆菌科的 MIC 为 45.6±20mg/ml，p<0.001。 芦荟叶提取物对 74 株金黄色葡萄球菌的平均 MBC 为 188±82.46mg/ml，对 73 株肠杆菌科的 MBC 为 91.18±40mg/ml，p<0.001。
芦荟乙醇叶提取物对引起伤口感染的不同细菌菌株表现出良好的抗菌作用。 本文显示了未来使用天然产品治疗伤口感染的可能性。
