Park Ui Jun, Kim Hyoung Tae, Cho Won Hyun, Park Jae Hyoung, Jung Hye Ra, Kim Min Young
Department of Surgery, Keimyung University School of Medicine, Daegu, Ulsan, Korea.
Department of Physiology, Keimyung University School of Medicine, Daegu, Ulsan, Korea.
Vasc Specialist Int. 2016 Dec;32(4):141-149. doi: 10.5758/vsi.2016.32.4.141. Epub 2016 Dec 31.
Ischemic preconditioning (IPC), including remote IPC (rIPC) and direct IPC (dIPC), is a promising method to decrease ischemia-reperfusion (IR) injury. This study tested the effect of both rIPC and dIPC on the genes for antioxidant enzymes and endoplasmic reticulum (ER) stress-related proteins.
Twenty rats were randomly divided into the control and study groups. In the control group (n=10), the right hind limb was sham-operated. The left hind limb (IscR) of the control group underwent IR injury without IPC. In the study group (n=10), the right hind limb received IR injury after 3 cycles of rIPC. The IscR received IR injury after 3 cycles of dIPC. Gene expression was analyzed by Quantitative real-time polymerase chain reaction from the anterior tibialis muscle.
The expression of the antioxidant enzyme genes including glutathione peroxidase (GPx), superoxide dismutase (SOD) 1 and catalase (CAT) were significantly reduced in IscR compared with sham treatment. In comparison with IscR, rIPC enhanced the expression of GPx, SOD2, and CAT genes. dIPC enhanced the expression of SOD2 and CAT genes. The expression of SOD2 genes was consistently higher in rIPC than in dIPC, but the difference was only significant for SOD2. The expression of genes for ER stress-related proteins tended to be reduced in IscR in comparison with sham treatment. However, the difference was only significant for C/EBP homologous protein (CHOP). In comparison with IscR, rIPC significantly up-regulated activating transcription factor 4 and CHOP, whereas dIPC up-regulated CHOP.
Both rIPC and dIPC enhanced expression of genes for antioxidant enzymes and ER stress-related proteins.
缺血预处理（IPC），包括远程缺血预处理（rIPC）和直接缺血预处理（dIPC），是一种有望减少缺血再灌注（IR）损伤的方法。本研究测试了rIPC和dIPC对抗氧化酶基因和内质网（ER）应激相关蛋白的影响。
将20只大鼠随机分为对照组和研究组。对照组（n = 10）右后肢进行假手术。对照组左后肢（IscR）接受无IPC的IR损伤。研究组（n = 10）右后肢在3个周期的rIPC后接受IR损伤。IscR在3个周期的dIPC后接受IR损伤。通过定量实时聚合酶链反应分析来自胫前肌的基因表达。
与假手术处理相比，IscR中包括谷胱甘肽过氧化物酶（GPx）、超氧化物歧化酶（SOD）1和过氧化氢酶（CAT）在内的抗氧化酶基因表达显著降低。与IscR相比，rIPC增强了GPx、SOD2和CAT基因的表达。dIPC增强了SOD2和CAT基因的表达。rIPC中SOD2基因的表达始终高于dIPC，但差异仅对SOD2有统计学意义。与假手术处理相比，IscR中ER应激相关蛋白的基因表达趋于降低。然而，差异仅对C/EBP同源蛋白（CHOP）有统计学意义。与IscR相比，rIPC显著上调激活转录因子4和CHOP，而dIPC上调CHOP。
rIPC和dIPC均增强了抗氧化酶基因和ER应激相关蛋白的表达。
