Hagag Adel A, Amin Saleh M, El-Fiky Rasha B, El-Sayad Magda E
Department of Pediatrics, Faculty of Medicine, Tanta University, Tanta, Egypt.
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tanta University, Tanta, Egypt.
Infect Disord Drug Targets. 2018;18(1):52-59. doi: 10.2174/1871526517666170102115116.
Helicobacter pylori are gram-negative spiral shaped bacteria, with sheathed flagella. H. pylori infection is one of the most common chronic infections in humans. Infection is usually acquired during childhood, and becomes a lifelong infection in most people unless treated. The aim of this study was to evaluate serum levels of oxidative stress indices in children with H. pylori infection.
The present study was carried out on 60 children infected with H. pylori including 28 males, 32 females with their age ranging from 7-10 years and mean age value of 8.5 ± 1.65 ( Group I). This study included also 60 children as a control group including 26 males, 34 females with their age ranging from 7-11 and mean age value of 8.99 ± 1.63 (Group II). For all children in groups I the following were done: Diagnosis of H. pylori infection through H. pylori stool antigen testing using enzyme immunoassay kit and gastric antrum mucosal biopsies which were tested for urease activity using Campylobacter like organism test (CLO test). Measurements of serum oxidative stress markers including Superoxide dismutase (SOD), Malondialdhyde, Glutathione, Catalase and Nitric oxide were done in patients and controls.
Serum nitric oxide and reduced glutathione were significantly lower in patients compared to controls while serum MDA, Serum catalase and Serum SOD were significantly higher in patients compared to controls (nitric oxide was 91.111 ±6.366 in patients versus 107.211±2.121 in controls with p value of 0.001, reduced glutathione in patients was 2.457± 0.081 versus 2.889±0.491 in controls with p value of 0.001, serum MDA in patients was 140.22±5.18 versus 116.22±2.98 in controls with p value of 0.001, catalase was 401.645± 4.344 versus 278.221±71.712 in controls with p value of 0.001 and SOD in patients was 16.936±9.145 versus 5.578±0.231 in controls with p value of 0.001).
H. pylori infection is associated with oxidative stress with significantly lower serum nitric oxide and reduced glutathione and significantly higher serum MDA, catalase and SOD in patients compared to controls.
Antioxidants may be beneficial adjuvant treatment in H. pylori infection as H. pylori infection is associated with oxidative stress.
幽门螺杆菌是革兰氏阴性螺旋形细菌，带有鞘鞭毛。幽门螺杆菌感染是人类最常见的慢性感染之一。感染通常在儿童时期获得，除非接受治疗，大多数人会终身感染。本研究的目的是评估幽门螺杆菌感染儿童的血清氧化应激指标水平。
本研究对60名感染幽门螺杆菌的儿童进行，其中男性28名，女性32名，年龄在7至10岁之间，平均年龄为8.5±1.65岁（第一组）。本研究还包括60名儿童作为对照组，其中男性26名，女性34名，年龄在7至11岁之间，平均年龄为8.99±1.63岁（第二组）。对第一组的所有儿童进行了以下操作：通过使用酶免疫分析试剂盒进行幽门螺杆菌粪便抗原检测以及通过弯曲菌样生物体试验（CLO试验）检测胃窦黏膜活检组织的尿素酶活性来诊断幽门螺杆菌感染。对患者和对照组进行血清氧化应激标志物的测量，包括超氧化物歧化酶（SOD）、丙二醛、谷胱甘肽、过氧化氢酶和一氧化氮。
与对照组相比，患者的血清一氧化氮和还原型谷胱甘肽显著降低，而患者的血清丙二醛、血清过氧化氢酶和血清超氧化物歧化酶显著升高（患者的一氧化氮为91.111±6.366，对照组为107.211±2.121，p值为0.001；患者的还原型谷胱甘肽为2.457±0.081，对照组为2.889±0.491，p值为0.001；患者的血清丙二醛为140.22±5.18，对照组为116.22±2.98，p值为0.001；过氧化氢酶为401.645±4.344，对照组为278.221±71.712，p值为0.001；患者的超氧化物歧化酶为16.936±9.145，对照组为5.578±0.231，p值为0.001）。
幽门螺杆菌感染与氧化应激相关，与对照组相比，患者的血清一氧化氮和还原型谷胱甘肽显著降低，血清丙二醛、过氧化氢酶和超氧化物歧化酶显著升高。
由于幽门螺杆菌感染与氧化应激相关，抗氧化剂可能是幽门螺杆菌感染有益的辅助治疗方法。
