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[吖啶橙在评估机体中淋巴细胞迁移方面的应用]

[Use of acridine orange in the estimation of lymphoid cell migration in an organism].

作者信息

Popugaĭlo M V, Nalivaĭko A M, Sharavara A A, Riabinin S R

出版信息

Biull Eksp Biol Med. 1989 Jul;108(7):40-2.

PMID:2804295
Abstract

Lymphoid cell migration was studied on the model of their transplantation from animal-donors to recipients. The studied organs were crushed small in the saline solution. The number of nuclei-containing elements has been determined in the obtained suspensions. Smears have been prepared and studied with the help of luminescence microscope combined with phase contrast device. We have determined and counted the ratio of "acridine orange cells" glittering in ultra-violet rays to all visible in phase-contrast nuclei containing cells. Then we have found the percentage of the marked cells in the organ from the total number of the injected lymphocytes. The usage of the given technique shown the enhancement of thymus cell migration to the liver after partial hepatectomy.

摘要

在淋巴细胞从动物供体移植到受体的模型上研究了淋巴细胞迁移。将研究的器官在盐溶液中碾碎成小块。已确定所得悬浮液中含核元素的数量。制备涂片并借助结合相差装置的发光显微镜进行研究。我们已确定并计算了在紫外线中发光的“吖啶橙细胞”与相差显微镜下所有可见的含细胞核细胞的比例。然后我们发现了标记细胞在器官中占注入淋巴细胞总数的百分比。使用该技术表明部分肝切除术后胸腺细胞向肝脏的迁移增强。

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