• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

NDRG4基因高甲基化是中国人群胃癌诊断和预后的潜在生物标志物。

NDRG4 hypermethylation is a potential biomarker for diagnosis and prognosis of gastric cancer in Chinese population.

作者信息

Chen Xiaoying, Yang Yong, Liu Jing, Li Bin, Xu Yan, Li Cong, Xu Qi, Liu Guili, Chen Yingmin, Ying Jieer, Duan Shiwei

机构信息

Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, China.

Department of Medical Oncology, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, China.

出版信息

Oncotarget. 2017 Jan 31;8(5):8105-8119. doi: 10.18632/oncotarget.14099.

DOI:10.18632/oncotarget.14099
PMID:28042954
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5352386/
Abstract

In order to assess whether N-Myc downstream regulated gene 4 (NDRG4) methylation was associated with the diagnosis and prognosis of gastric cancer, we measured the methylation of NDRG4 promoter and gene body regions among 110 gastric cancer patients using quantitative methods (MethyLight and pyrosequencing). Both NDRG4 promoter and gene body methylation levels were increased in tumor tissues than paired adjacent normal tissues (P < 0.001). NDRG4 gene body methylation was found to be significantly associated with age and tumor differentiation. NDRG4 promoter hypermethylation was proved to be a predictor of poor overall survival. However, opposite result was observed among The Cancer Genome Atlas (TCGA) cohort. The findings from gastric cell lines and public databases have suggested that NDRG4 methylation level was inversely associated with NDRG4 transcription level. Subsequent luciferase reporter gene assay showed that promoter CpG island but not gene body CpG island was able to upregulate gene expression. Collectively, NDRG4 promoter hypermethylation contributed to the risk of gastric cancer and predicted a poor prognosis in Chinese gastric cancer patients. Moreover, the combined methylation levels of NDRG4 promoter and gene body served as diagnostic biomarkers in gastric cancer.

摘要

为了评估N-Myc下游调控基因4（NDRG4）甲基化与胃癌的诊断及预后是否相关，我们采用定量方法（甲基化特异性荧光定量PCR和焦磷酸测序）检测了110例胃癌患者中NDRG4启动子和基因体区域的甲基化情况。与配对的癌旁正常组织相比，肿瘤组织中NDRG4启动子和基因体的甲基化水平均升高（P < 0.001）。发现NDRG4基因体甲基化与年龄和肿瘤分化显著相关。NDRG4启动子高甲基化被证明是总生存期较差的一个预测指标。然而，在癌症基因组图谱（TCGA）队列中观察到了相反的结果。来自胃癌细胞系和公共数据库的研究结果表明，NDRG4甲基化水平与NDRG4转录水平呈负相关。随后的荧光素酶报告基因实验表明，启动子CpG岛而非基因体CpG岛能够上调基因表达。总体而言，NDRG4启动子高甲基化增加了胃癌风险，并预测中国胃癌患者预后不良。此外，NDRG4启动子和基因体的联合甲基化水平可作为胃癌的诊断生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20ef/5352386/20e69206d0ff/oncotarget-08-8105-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20ef/5352386/fd3d07fa3de6/oncotarget-08-8105-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20ef/5352386/d3dd6799e55d/oncotarget-08-8105-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20ef/5352386/c23b4df83754/oncotarget-08-8105-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20ef/5352386/e2ab59b7e476/oncotarget-08-8105-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20ef/5352386/20e69206d0ff/oncotarget-08-8105-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20ef/5352386/fd3d07fa3de6/oncotarget-08-8105-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20ef/5352386/d3dd6799e55d/oncotarget-08-8105-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20ef/5352386/c23b4df83754/oncotarget-08-8105-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20ef/5352386/e2ab59b7e476/oncotarget-08-8105-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20ef/5352386/20e69206d0ff/oncotarget-08-8105-g005.jpg

相似文献

1
NDRG4 hypermethylation is a potential biomarker for diagnosis and prognosis of gastric cancer in Chinese population.NDRG4基因高甲基化是中国人群胃癌诊断和预后的潜在生物标志物。
Oncotarget. 2017 Jan 31;8(5):8105-8119. doi: 10.18632/oncotarget.14099.
2
NDRG4 in gastric cancer determines tumor cell proliferation and clinical outcome.NDRG4 在胃癌中决定肿瘤细胞增殖和临床结局。
Mol Carcinog. 2018 Jun;57(6):762-771. doi: 10.1002/mc.22798. Epub 2018 Mar 24.
3
N-Myc downstream-regulated gene 4 (NDRG4): a candidate tumor suppressor gene and potential biomarker for colorectal cancer.N-Myc下游调控基因4（NDRG4）：一种结直肠癌的候选抑癌基因和潜在生物标志物。
J Natl Cancer Inst. 2009 Jul 1;101(13):916-27. doi: 10.1093/jnci/djp131. Epub 2009 Jun 17.
4
TFPI2 and NDRG4 gene promoter methylation analysis in peripheral blood mononuclear cells are novel epigenetic noninvasive biomarkers for colorectal cancer diagnosis.外周血单个核细胞中 TFPI2 和 NDRG4 基因启动子甲基化分析是结直肠癌诊断的新型表观遗传非侵入性生物标志物。
J Gene Med. 2020 Aug;22(8):e3189. doi: 10.1002/jgm.3189. Epub 2020 Apr 14.
5
Odd-skipped related 1 is a novel tumour suppressor gene and a potential prognostic biomarker in gastric cancer.奇跳相关蛋白1是一种新型肿瘤抑制基因，也是胃癌潜在的预后生物标志物。
J Pathol. 2014 Nov;234(3):302-15. doi: 10.1002/path.4391. Epub 2014 Aug 28.
6
Hypermethylation-mediated silencing of NDRG4 promotes pancreatic ductal adenocarcinoma by regulating mitochondrial function.NDRG4 的高甲基化介导的沉默通过调节线粒体功能促进胰腺导管腺癌。
BMB Rep. 2020 Dec;53(12):658-663. doi: 10.5483/BMBRep.2020.53.12.168.
7
Promoter methylation and expression of TIMP3 gene in gastric cancer.胃癌中 TIMP3 基因的启动子甲基化和表达。
Diagn Pathol. 2013 Jul 2;8:110. doi: 10.1186/1746-1596-8-110.
8
Identification of functional methylated CpG loci in PD-L1 promoter as the novel epigenetic biomarkers for primary gastric cancer.鉴定 PD-L1 启动子中功能性甲基化 CpG 位点作为原发性胃癌的新型表观遗传生物标志物。
Gene. 2021 Mar 10;772:145376. doi: 10.1016/j.gene.2020.145376. Epub 2020 Dec 23.
9
Detection of RASSF1A promoter hypermethylation in serum from gastric and colorectal adenocarcinoma patients.胃癌和结直肠癌患者血清中RASSF1A启动子高甲基化的检测
World J Gastroenterol. 2008 May 21;14(19):3074-80. doi: 10.3748/wjg.14.3074.
10
GFRA3 promoter methylation may be associated with decreased postoperative survival in gastric cancer.胶质细胞源性神经营养因子受体α3（GFRA3）启动子甲基化可能与胃癌术后生存率降低有关。
BMC Cancer. 2016 Mar 16;16:225. doi: 10.1186/s12885-016-2247-8.

引用本文的文献

1
Comprehensive Multiomics Analyses Establish the Optimal Prognostic Model for Resectable Gastric Cancer : Prognosis Prediction for Resectable GC.全面多组学分析为可切除胃癌建立了最佳预后模型：可切除 GC 的预后预测。
Ann Surg Oncol. 2024 Mar;31(3):2078-2089. doi: 10.1245/s10434-023-14249-x. Epub 2023 Nov 23.
2
Bioinformatics Prediction and Machine Learning on Gene Expression Data Identifies Novel Gene Candidates in Gastric Cancer.基于基因表达数据的生物信息学预测和机器学习鉴定胃癌新的候选基因。
Genes (Basel). 2022 Nov 28;13(12):2233. doi: 10.3390/genes13122233.
3
NDRG4 sensitizes CRC cells to 5-FU by upregulating DDIT3 expression.

本文引用的文献

1
Multimodal approach of advanced gastric cancer: based therapeutic algorithm.晚期胃癌的多模式治疗方法：基于治疗的算法。
Eur Rev Med Pharmacol Sci. 2016 Oct;20(19):4018-4031.
2
Etiology and Prevention of Gastric Cancer.胃癌的病因与预防
Gastrointest Tumors. 2016 Sep;3(1):25-36. doi: 10.1159/000443995. Epub 2016 Feb 12.
3
Genome-scale methylation assessment did not identify prognostic biomarkers in oral tongue carcinomas.全基因组甲基化评估未在口腔舌癌中鉴定出预后生物标志物。
NDRG4通过上调DDIT3表达使结直肠癌细胞对5-氟尿嘧啶敏感。
Oncol Lett. 2021 Nov;22(5):782. doi: 10.3892/ol.2021.13043. Epub 2021 Sep 13.
4
Identification of novel prognostic biomarkers by integrating multi-omics data in gastric cancer.通过整合胃癌多组学数据鉴定新型预后生物标志物。
BMC Cancer. 2021 Apr 26;21(1):460. doi: 10.1186/s12885-021-08210-y.
5
IL10 hypomethylation is associated with the risk of gastric cancer.白细胞介素10（IL10）低甲基化与胃癌风险相关。
Oncol Lett. 2021 Apr;21(4):241. doi: 10.3892/ol.2021.12502. Epub 2021 Jan 31.
6
Hypermethylation-mediated silencing of NDRG4 promotes pancreatic ductal adenocarcinoma by regulating mitochondrial function.NDRG4 的高甲基化介导的沉默通过调节线粒体功能促进胰腺导管腺癌。
BMB Rep. 2020 Dec;53(12):658-663. doi: 10.5483/BMBRep.2020.53.12.168.
7
The values of AHCY and CBS promoter methylation on the diagnosis of cerebral infarction in Chinese Han population.AHCY 和 CBS 启动子甲基化在汉族人群脑梗死诊断中的价值。
BMC Med Genomics. 2020 Nov 2;13(1):163. doi: 10.1186/s12920-020-00798-7.
8
N-MYC Downstream Regulated Gene 4 (), a Frequent Downregulated Gene through DNA Hypermethylation, plays a Tumor Suppressive Role in Esophageal Adenocarcinoma.N-MYC下游调控基因4（NDRG4）是一种通过DNA高甲基化频繁下调的基因，在食管腺癌中发挥肿瘤抑制作用。
Cancers (Basel). 2020 Sep 10;12(9):2573. doi: 10.3390/cancers12092573.
9
Non-Invasive Colorectal Cancer Screening: An Overview.非侵入性结直肠癌筛查：概述
Gastrointest Tumors. 2020 Jul;7(3):62-73. doi: 10.1159/000507701. Epub 2020 May 20.
10
Role of DNA Methylation in the Resistance to Therapy in Solid Tumors.DNA甲基化在实体瘤治疗耐药中的作用
Front Oncol. 2020 Aug 7;10:1152. doi: 10.3389/fonc.2020.01152. eCollection 2020.
Clin Epigenetics. 2016 Jul 18;8:74. doi: 10.1186/s13148-016-0235-0. eCollection 2016.
4
Clinical significance and diagnostic value of serum CEA, CA19-9 and CA72-4 in patients with gastric cancer.血清癌胚抗原、糖类抗原19-9及糖类抗原72-4在胃癌患者中的临床意义及诊断价值
Oncotarget. 2016 Aug 2;7(31):49565-49573. doi: 10.18632/oncotarget.10391.
5
Validation of DAB2IP methylation and its relative significance in predicting outcome in renal cell carcinoma.DAB2IP甲基化在预测肾细胞癌预后中的验证及其相对意义
Oncotarget. 2016 May 24;7(21):31508-19. doi: 10.18632/oncotarget.8971.
6
Field Cancerization in Sporadic Colon Cancer.散发性结肠癌中的场癌化
Gut Liver. 2016 Sep 15;10(5):773-80. doi: 10.5009/gnl15334.
7
Cancer statistics in China, 2015.《中国癌症统计数据 2015》
CA Cancer J Clin. 2016 Mar-Apr;66(2):115-32. doi: 10.3322/caac.21338. Epub 2016 Jan 25.
8
SSTR2 promoter hypermethylation is associated with the risk and progression of laryngeal squamous cell carcinoma in males.生长抑素受体2（SSTR2）启动子高甲基化与男性喉鳞状细胞癌的风险及进展相关。
Diagn Pathol. 2016 Jan 22;11:10. doi: 10.1186/s13000-016-0461-y.
9
Significance of Serum Pepsinogens as a Biomarker for Gastric Cancer and Atrophic Gastritis Screening: A Systematic Review and Meta-Analysis.血清胃蛋白酶原作为胃癌和萎缩性胃炎筛查生物标志物的意义：一项系统评价和荟萃分析
PLoS One. 2015 Nov 10;10(11):e0142080. doi: 10.1371/journal.pone.0142080. eCollection 2015.
10
DNA Methylation as Surrogate Marker For Gastric Cancer.DNA甲基化作为胃癌的替代标志物
J Cancer Prev. 2015 Sep;20(3):172-8. doi: 10.15430/JCP.2015.20.3.172.