胃癌中与 microRNA-148b 相关的长非编码 RNA 的微阵列分析。

Microarray analysis of long non-coding RNAs related to microRNA-148b in gastric cancer.

出版信息

Neoplasma. 2017;64(2):199-208. doi: 10.4149/neo_2017_205.

DOI:10.4149/neo_2017_205

Abstract

The miR-148/152 family (miR-148a, miR-148b, miR-152) is differentially expressed in gastric cancer tissues and non-tumor tissues. Our previous studies indicated that miR-148/152 family is important in the tumorigenesis and development of gastric cancer. We also found several target genes that were regulated by the miR-148/152 family and several factors that could influence members of this family. However, the molecular mechanisms and function of this family in gastric cancer remain unclear. Recently, long non-coding RNAs (lncRNAs) have been found to play an important role in the pathogenesis of many diseases. The aim of the present study was to perform expression profiling to identify lncRNAs that might be associated with miR-148b in gastric cancer and predict their potential functions. Total RNA was extracted from gastric cancer cell line SGC-7901 treated with miR-148b mimics and from untreated gastric cancer cells. Microarray analysis was performed using the Agilent LncRNA + mRNA Human Gene Expression Microarray V3.0 platform, which was designed for the profiling of human lncRNAs and protein-coding transcripts. A total of 37581 lncRNA and 34303 mRNA transcripts were detected in gastric cancer cell line SGC-7901 and cell line SGC-7901 treated with miR-148b mimics. Among the differentially expressed lncRNAs, 240 up-regulated and 64 down-regulated lncRNAs were identified. XLOC_000983 was the most up-regulated lncRNA, and M18204.1 was the most down-regulated lncRNA. Among the differentially expressed mRNAs in different cell lines, 196 were consistently up-regulated and 320 were consistently down-regulated. POSTN was the most up-regulated mRNA, and HBB was the most down-regulated mRNA. Gene Ontology (GO) and pathway analyses indicated that the lncRNAs influenced by miR-148b might play an important role in the immune system and were associated with the development of gastric cancer. The altered expression of lncRNAs influenced by miR-148b may play a partial role in pathways implicated in gastric cancer development and progression, such as the immune response pathway.

摘要

miR-148/152 家族（miR-148a、miR-148b、miR-152）在胃癌组织和非肿瘤组织中表达差异。我们的先前研究表明，miR-148/152 家族在胃癌的发生和发展中起重要作用。我们还发现了一些受 miR-148/152 家族调控的靶基因和一些可以影响该家族成员的因素。然而，该家族在胃癌中的分子机制和功能仍不清楚。最近，长非编码 RNA（lncRNA）已被发现在许多疾病的发病机制中发挥重要作用。本研究旨在进行表达谱分析，以鉴定与胃癌中 miR-148b 相关的 lncRNA，并预测其潜在功能。从用 miR-148b 模拟物处理的胃癌细胞系 SGC-7901 和未经处理的胃癌细胞中提取总 RNA。使用 Agilent LncRNA + mRNA Human Gene Expression Microarray V3.0 平台进行微阵列分析，该平台专为人类 lncRNA 和蛋白编码转录本的分析而设计。在胃癌细胞系 SGC-7901 和用 miR-148b 模拟物处理的细胞系 SGC-7901 中检测到 37581 个 lncRNA 和 34303 个 mRNA 转录本。在差异表达的 lncRNA 中，鉴定出 240 个上调和 64 个下调的 lncRNA。XLOC_000983 是上调最明显的 lncRNA，而 M18204.1 是下调最明显的 lncRNA。在不同细胞系中差异表达的 mRNA 中，196 个一致上调，320 个一致下调。POSTN 是上调最明显的 mRNA，HBB 是下调最明显的 mRNA。基因本体论（GO）和通路分析表明，受 miR-148b 影响的 lncRNA 可能在免疫系统中发挥重要作用，并与胃癌的发生有关。受 miR-148b 影响的 lncRNA 的表达改变可能在胃癌发展和进展涉及的途径中发挥部分作用，例如免疫反应途径。