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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Hudson G H, Omprakash A V, Premavalli K, Dhinakar Raj G

a Department of Poultry Science, Madras Veterinary College , Tamil Nadu Veterinary and Animal Sciences University , Chennai , India.

b Poultry Research Station , Tamil Nadu Veterinary and Animal Sciences University , Chennai , India.

Br Poult Sci. 2017 Apr;58(2):192-199. doi: 10.1080/00071668.2016.1262004. Epub 2017 Jan 24.

Guinea fowl hens were inseminated weekly once with two doses of spermatozoa (75 million and 100 million) in two different diluents, Beltsville poultry semen extender (BPSE), and Instruments for Veterinary Medicine (IMV), each with and without pre-insemination vaginal douching. Per cent fertility, hatchability, dead germ, dead in shells along with data on sperm egg interaction and vaginal microbial counts were recorded. 2. Artificial insemination had significantly improved the per cent fertility and hatchability compared to natural mating. Dose dependent improvement in fertility was noticed with both diluents. 3. There was a beneficial effect of vaginal douching, which was more pronounced at lower insemination doses. 4. For optimum fertility and hatchability in guinea fowl, insemination of 75 million spermatozoa diluted in BPSE once in 4 d and 100 million spermatozoa diluted in BPSE or IMV once in 5 d coupled with vaginal douching is recommended.

每周用两种不同稀释液（贝尔茨维尔家禽精液稀释剂（BPSE）和兽用器械公司（IMV）的稀释液）中的两种精子剂量（7500万和1亿）对珍珠鸡母鸡进行一次人工授精，每种稀释液在授精前有无阴道冲洗。记录受精率、孵化率、死胚率、壳内死亡情况以及精子与卵子相互作用和阴道微生物计数的数据。2. 与自然交配相比，人工授精显著提高了受精率和孵化率。两种稀释液都观察到了受精率的剂量依赖性提高。3. 阴道冲洗有有益效果，在较低授精剂量时更为明显。4. 为使珍珠鸡获得最佳受精率和孵化率，建议每4天用BPSE稀释的7500万个精子进行一次授精，每5天用BPSE或IMV稀释的1亿个精子进行一次授精，并进行阴道冲洗。

Hudson G H, Omprakash A V, Premavalli K, Dhinakar Raj G

a Department of Poultry Science, Madras Veterinary College , Tamil Nadu Veterinary and Animal Sciences University , Chennai , India.

b Poultry Research Station , Tamil Nadu Veterinary and Animal Sciences University , Chennai , India.

Br Poult Sci. 2017 Apr;58(2):192-199. doi: 10.1080/00071668.2016.1262004. Epub 2017 Jan 24.

Guinea fowl hens were inseminated weekly once with two doses of spermatozoa (75 million and 100 million) in two different diluents, Beltsville poultry semen extender (BPSE), and Instruments for Veterinary Medicine (IMV), each with and without pre-insemination vaginal douching. Per cent fertility, hatchability, dead germ, dead in shells along with data on sperm egg interaction and vaginal microbial counts were recorded. 2. Artificial insemination had significantly improved the per cent fertility and hatchability compared to natural mating. Dose dependent improvement in fertility was noticed with both diluents. 3. There was a beneficial effect of vaginal douching, which was more pronounced at lower insemination doses. 4. For optimum fertility and hatchability in guinea fowl, insemination of 75 million spermatozoa diluted in BPSE once in 4 d and 100 million spermatozoa diluted in BPSE or IMV once in 5 d coupled with vaginal douching is recommended.

每周用两种不同稀释液（贝尔茨维尔家禽精液稀释剂（BPSE）和兽用器械公司（IMV）的稀释液）中的两种精子剂量（7500万和1亿）对珍珠鸡母鸡进行一次人工授精，每种稀释液在授精前有无阴道冲洗。记录受精率、孵化率、死胚率、壳内死亡情况以及精子与卵子相互作用和阴道微生物计数的数据。2. 与自然交配相比，人工授精显著提高了受精率和孵化率。两种稀释液都观察到了受精率的剂量依赖性提高。3. 阴道冲洗有有益效果，在较低授精剂量时更为明显。4. 为使珍珠鸡获得最佳受精率和孵化率，建议每4天用BPSE稀释的7500万个精子进行一次授精，每5天用BPSE或IMV稀释的1亿个精子进行一次授精，并进行阴道冲洗。