Sexton T J
US Department of Agriculture, Avian Physiology Laboratory, Beltsville, Maryland 20705.
Poult Sci. 1988 Jan;67(1):131-4. doi: 10.3382/ps.0670131.
The ability was examined of three commercial turkey semen diluents, Beltsville Poultry Semen Extender II (BPSE), Instruments for Veterinary Medicine (IMV), and Minnesota Turkey Growers Association (MTGA) and two diluents containing antibiotics, BPSE + tobramycin (T) and MTGA + gentamicin (G), to maintain the fertilizing capacity of turkey semen held for 24 h at 5 C. Hens were inseminated weekly with 200 million viable spermatozoa for 15 wk. Fertility of unstored, diluted (1:1) semen in MTGA + G (90%) was significantly lower than semen diluted in BPSE (95%) or IMV (95%). Fertilizing capacity of spermatozoa diluted in BPSE + T (94%) and MTGA (91%) was not different from that of semen diluted in the other diluents. Fertility of semen stored for 24 h was highest stored in BPSE (77%) followed by values for storage in BPSE + T (64%), MTGA + G (60%), MTGA (41%), and IMV (38%). Fertility was affected by a significant interaction between semen diluent and storage time. When compared with effects of storage in both Beltsville extenders, spermatozoa stored in the other diluents were less motile. Spermatozoa stored in all extenders except BPSE had a high rate of lysis.
对三种市售火鸡精液稀释剂，即贝尔茨维尔家禽精液稀释剂II（BPSE）、兽用器械公司（IMV）和明尼苏达火鸡养殖者协会（MTGA）以及两种含抗生素的稀释剂，即BPSE + 妥布霉素（T）和MTGA + 庆大霉素（G），维持在5℃保存24小时的火鸡精液受精能力的情况进行了检测。母鸡每周用2亿个活精子进行人工授精，持续15周。未保存的、用MTGA + G稀释（1:1）的精液受精率（90%）显著低于用BPSE（95%）或IMV（95%）稀释的精液。用BPSE + T（94%）和MTGA（91%）稀释的精子受精能力与用其他稀释剂稀释的精液无差异。保存24小时的精液受精率最高的是保存在BPSE中的（77%），其次是保存在BPSE + T中的（64%）、MTGA + G中的（60%）、MTGA中的（41%）和IMV中的（38%）。受精率受精液稀释剂和保存时间之间显著相互作用的影响。与保存在两种贝尔茨维尔稀释剂中的效果相比，保存在其他稀释剂中的精子活力较低。保存在除BPSE之外所有稀释剂中的精子裂解率较高。
