Sun X C, Zhang F, Zhang W, Shao H, Wang Z X
School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Shandong Institute of Prevention and Control of Occupational Disease, Jinan250062, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2016 Nov 20;34(11):810-813. doi: 10.3760/cma.j.issn.1001-9391.2016.11.003.
To investigate the influence of genetic polymorphisms of GSTT1 and GSTM1 on metabolism of polycyclic aromatic hydrocarbon (PAHs) in coal tar pitch workers and to explore the effective bio-marker of occupational exposure to coal tar pitch. 374 cases of workers exposed to coal tar pitch work at the enterprise which use and product coal tar pitch in Shan Dong province named as exposure group.The ad-ministrative and the support staff 135 cases were selected as control group.The concentration of the metabolic product in urine by HPLC/MC.The genetic polymorphisms of GSTT1 and GSTM1 were detected by multiple PCR method. The content of metabolic product in smoke group were significantly higher than workers do not smoke (<0.05) . The content of metabolic product in exposure group were significantly higher than control group (<0.05) . The content of metabolic product in workers carry positive GSTT1 and GSTM1 were significantly high-er than workers carry negative one (<0.05) . The content of metabolic product in different types of labor were significantly different (<0.05) . The content of metabolic product were significantly higher in urine after work than before work (<0.05) . The content of metabolic product and workers carry positive GSTT1 and GSTM1 had a positive correlation (<0.05) . Carrying positive GSTT1 and GSTM1 promote polycyclic aromatic hydrocarbons in the body's metabolism.
探讨谷胱甘肽硫转移酶T1（GSTT1）和谷胱甘肽硫转移酶M1（GSTM1）基因多态性对煤焦油沥青作业工人多环芳烃（PAHs）代谢的影响，探索煤焦油沥青职业接触的有效生物标志物。选取山东省某生产和使用煤焦油沥青企业的374名煤焦油沥青接触工人作为暴露组，选取135名行政及后勤人员作为对照组。采用高效液相色谱/质谱联用法检测尿中代谢产物浓度，采用多重聚合酶链反应法检测GSTT1和GSTM1基因多态性。吸烟组代谢产物含量显著高于不吸烟工人（<0.05）。暴露组代谢产物含量显著高于对照组（<0.05）。携带GSTT1和GSTM1阳性的工人代谢产物含量显著高于携带阴性的工人（<0.05）。不同工种的代谢产物含量有显著差异（<0.05）。下班后尿中代谢产物含量显著高于上班前（<0.05）。代谢产物含量与携带GSTT1和GSTM1阳性的工人呈正相关（<0.05）。携带GSTT1和GSTM1阳性促进体内多环芳烃的代谢。
