de Campos Felipe Terres, Rincon Joao Aveiro Alvarado, Acosta Diego Andres Velasco, Silveira Pedro Augusto Silva, Pradieé Jorgea, Corrêa Marcio Nunes, Gasperin Bernardo Garziera, Pfeifer Luiz Francisco Machado, Barros Carlos Castilho, Pegoraro Ligia Margareth Cantareli, Schneider Augusto
Department of Pathology, Veterinary College, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
Department of Veterinary Clinics, Veterinary College, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
Theriogenology. 2017 Feb;89:244-249. doi: 10.1016/j.theriogenology.2016.11.013. Epub 2016 Nov 18.
The aim of this study was to evaluate the effect of an acute systemic inflammatory response induced by lipopolysaccharide (LPS) in the serum and follicular fluid (FF) high-density lipoprotein (HDL) components, hormone concentrations and granulosa cell gene expression. For this purpose, twenty non-lactating Jersey dairy cows were submitted to a progesterone (P4) - estradiol (E2) based synchronization protocol. Cows received a single i.v. dose of LPS (2.5 μg/kg of body weight) or saline solution (CTL Group) 2 h after P4 insert removal. Blood, granulosa cells and FF samples were collected six hours after LPS injection. Five hours after LPS injection rectal temperature was increased in LPS (P < 0.0001, 40.4 ± 0.1 °C) compared to the CTL cows (38.8 ± 0.1 °C). Serum PON1 activity was reduced by LPS injection (130.2 ± 5.1 vs. 99.6 ± 3.3 U/mL; P < 0.001), as well as HDL-cholesterol concentrations (70.3 ± 5.3 vs. 50.1 ± 6.2 mg/dL; P < 0.05). The FF E2 and P4 concentrations were not different between groups (P > 0.05). The PON1 activity in the FF was also decreased by LPS injection (P = 0.01). In comparison to CTL group, cows injected with LPS had a ten fold reduction in STAR, TLR4 and TNF mRNA expression (P < 0.05). In conclusion, an intravenous LPS challenge in cows induced an acute systemic inflammatory response reducing HDL and its components in serum but not in the FF. Only PON1 activity serum reduction was reflected in the FF in the short term. Additionally, steroidogenic and inflammatory genes had reduced expression in the granulosa cells.
本研究的目的是评估脂多糖（LPS）诱导的急性全身炎症反应对血清和卵泡液（FF）中高密度脂蛋白（HDL）成分、激素浓度以及颗粒细胞基因表达的影响。为此，对20头非泌乳泽西奶牛采用基于孕酮（P4）-雌二醇（E2）的同期发情方案。在取出P4植入物2小时后，奶牛接受单次静脉注射LPS（2.5μg/kg体重）或生理盐水（CTL组）。LPS注射6小时后采集血液、颗粒细胞和FF样本。与CTL组奶牛（38.8±0.1°C）相比，LPS组奶牛在LPS注射5小时后直肠温度升高（P<0.0001，40.4±0.1°C）。LPS注射使血清对氧磷酶1（PON1）活性降低（130.2±5.1对99.6±3.3 U/mL；P<0.001），高密度脂蛋白胆固醇浓度也降低（70.3±5.3对50.1±6.2 mg/dL；P<0.05）。两组间FF中E2和P4浓度无差异（P>0.05）。LPS注射也使FF中PON1活性降低（P=0.01）。与CTL组相比，注射LPS的奶牛中类固醇生成急性调节蛋白（STAR）、Toll样受体4（TLR4）和肿瘤坏死因子（TNF）mRNA表达降低了10倍（P<0.05）。总之，奶牛静脉注射LPS激发了急性全身炎症反应，降低了血清中HDL及其成分，但未影响FF中的这些指标。短期内，只有血清PON1活性的降低反映在FF中。此外，颗粒细胞中类固醇生成和炎症相关基因的表达降低。
