Hussain Shah S Aftab, Hassan Andrabi S Murtaza, Ahmed Hussain, Qureshi Irfan Zia
Animal Reproduction Laboratory, Animal Sciences Institute, National Agricultural Research Center, Islamabad, Pakistan; Laboratory of Animal and Human Physiology, Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Animal Reproduction Laboratory, Animal Sciences Institute, National Agricultural Research Center, Islamabad, Pakistan.
Theriogenology. 2017 Feb;89:32-40. doi: 10.1016/j.theriogenology.2016.10.009. Epub 2016 Oct 19.
This study was primarily designed to evaluate the effect of different concentrations of ultraviolet (UV)-C-irradiated chicken egg yolk plasma (EYP; v:v; 10%, P1; 15%, P2; 20%, P3) or 20% (v:v) of whole chicken egg yolk (WCEY) in tris-citric acid (TCA) extender on water buffalo sperm quality during cryopreservation (postdilution, PD; postequilibration, PE; post-thawing, PT). Also the effect of best evolved concentration of UV-C-irradiated EYP in extender on in vivo fertility of buffalo spermatozoa was evaluated. At PE and PT, computer-assisted sperm analysis progressive motility (PM, %) was significantly higher in P3 compared with P1 and WCEY. Rapid velocity (RV, %) was higher (P < 0.05) in P3 compared with P1 and WCEY during cryopreservation (PD, PE, and PT). Average path velocity (μm/s) and straight line velocity (μm/s) were higher (P < 0.05) in P2 and P3 than WCEY at PE and PT. The decline percentage (%, longevity) in PM and RV was lower (P < 0.05) in P3 compared with WCEY during 2 hours incubation under in vitro condition at PT. Supravital plasma membrane integrity (%) was higher (P < 0.05) in P2 and P3 compared with control at different stages (PE and PT). Mitochondrial transmembrane potential (%) was higher (P < 0.05) in P2 and P3 compared with P1 and WCEY at different stages (PD and PT). Percentage of viable sperm with intact acrosome, and sperm DNA integrity (%) were higher (P < 0.05) in P2 and P3 compared with WCEY at PT. The in vivo fertility rate (%) was significantly higher with P3 compared with WCEY (76.61 vs. 64.49). In conclusion, WCEY (20%) can be replaced with UV-C-irradiated chicken EYP (20%) in TCA extender for cryopreservation of water buffalo spermatozoa.
本研究主要旨在评估不同浓度的紫外线（UV）-C照射的鸡蛋黄血浆（EYP；体积比：10%，P1；15%，P2；20%，P3）或20%（体积比）的全鸡蛋黄（WCEY）添加到柠檬酸三钠（TCA）稀释液中，对水牛精子冷冻保存过程（稀释后，PD；平衡后，PE；解冻后，PT）中精子质量的影响。同时评估了稀释液中UV-C照射的EYP最佳浓度对水牛精子体内受精能力的影响。在PE和PT阶段，与P1和WCEY相比，P3组的计算机辅助精子分析渐进性运动率（PM，%）显著更高。在冷冻保存期间（PD、PE和PT），与P1和WCEY相比，P3组的快速运动速度（RV，%）更高（P<0.05）。在PE和PT阶段，P2和P3组的平均路径速度（μm/s）和直线速度（μm/s）高于WCEY组（P<0.05）。在PT阶段体外培养2小时期间，与WCEY相比，P3组的PM和RV下降百分比（%，存活时间）更低（P<0.05）。在不同阶段（PE和PT），与对照组相比，P2和P3组的超活细胞膜完整性（%）更高（P<0.05）。在不同阶段（PD和PT），与P1和WCEY相比，P2和P3组的线粒体跨膜电位（%）更高（P<0.05）。在PT阶段，与WCEY相比，P2和P3组具有完整顶体的活精子百分比和精子DNA完整性（%）更高（P<0.05）。与WCEY相比，P3组的体内受精率（%）显著更高（76.61对64.49）。总之，在TCA稀释液中，20%的WCEY可以被UV-C照射的鸡蛋黄EYP（20%）替代用于水牛精子的冷冻保存。
