多房棘球绦虫基因组上编码的假定丝氨酸蛋白酶抑制剂（serpin）基因及其在中绦期的表达。
The putative serine protease inhibitor (serpin) genes encoded on Echinococcus multilocularis genome and their expressions in metacestodal stage.
作者信息
Sasaki Mizuki, Sako Yasuhito
机构信息
Department of Parasitology, Asahikawa Medical University, 2-1-1-1, Midorigaoka-Higashi, Asahikawa, Hokkaido, 078-8510, Japan.
Department of Parasitology, Asahikawa Medical University, 2-1-1-1, Midorigaoka-Higashi, Asahikawa, Hokkaido, 078-8510, Japan.
出版信息
Vet Parasitol. 2017 Jan 15;233:20-24. doi: 10.1016/j.vetpar.2016.11.019. Epub 2016 Nov 27.
Two putative serpin genes were identified in Echinococcus multilocularis, in addition to the already reported serpinEmu, and were designated as serpin2Emu and serpin3Emu. Western blot analysis using polyclonal antibodies against serpinEmu, putative serpin2Emu protein, and putative serpin3Emu protein indicated that all three proteins were localized in both intracellular and excretory-secretory (ES) fractions of E. multilocularis metacestodes. In addition, immune staining of parasite tissue indicated that all three proteins were localized at the germinal layer.
除了已报道的丝氨酸蛋白酶抑制剂Emu（serpinEmu）外，在多房棘球绦虫中还鉴定出两个假定的丝氨酸蛋白酶抑制剂基因，分别命名为丝氨酸蛋白酶抑制剂2Emu（serpin2Emu）和丝氨酸蛋白酶抑制剂3Emu（serpin3Emu）。使用针对丝氨酸蛋白酶抑制剂Emu、假定的丝氨酸蛋白酶抑制剂2Emu蛋白和假定的丝氨酸蛋白酶抑制剂3Emu蛋白的多克隆抗体进行的蛋白质免疫印迹分析表明，这三种蛋白质均定位于多房棘球绦虫成虫囊泡的细胞内和排泄-分泌（ES）组分中。此外，对寄生虫组织的免疫染色表明，这三种蛋白质均定位于生发层。
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