Valot Benoît, Knapp Jenny, Umhang Gérald, Grenouillet Frédéric, Millon Laurence
Chrono-environnement Laboratory, UMR 6249 CNRS, University of Franche-Comté, 16 route de Gray, F-25030 Besançon, France.
Chrono-environnement Laboratory, UMR 6249 CNRS, University of Franche-Comté, 16 route de Gray, F-25030 Besançon, France.
Infect Genet Evol. 2015 Jun;32:338-41. doi: 10.1016/j.meegid.2015.03.040. Epub 2015 Apr 4.
EmsB is a molecular marker applied to Echinococcus multilocularis genotyping studies. This marker has largely been used to investigate the epidemiology of the parasite in different endemic foci. The present study has lifted the veil on the genetic structure of this microsatellite. By in silico analysis on the E. multilocularis genome the microsatellite was described in about 40 copies on the chromosome 5 of the parasite. Similar structure was found in the relative parasite Echinococcus granulosus, where the microsatellite was firstly described. The present study completes the first investigations made on the EmsB microsatellite origins and confirms the reliability of this highly discriminant molecular marker.
EmsB是一种应用于多房棘球绦虫基因分型研究的分子标记。该标记主要用于调查不同流行病灶中该寄生虫的流行病学情况。本研究揭开了这种微卫星的遗传结构面纱。通过对多房棘球绦虫基因组的电子分析，发现该微卫星在寄生虫的5号染色体上约有40个拷贝。在相关寄生虫细粒棘球绦虫中也发现了类似结构，微卫星最初就是在细粒棘球绦虫中被描述的。本研究完成了对EmsB微卫星起源的首次调查，并证实了这种高度鉴别性分子标记的可靠性。
