Durnam D M, Anders K R, Fisher L, O'Quigley J, Bryant E M, Thomas E D
Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
Blood. 1989 Nov 1;74(6):2220-6.
A Y-chromosome-specific in situ hybridization assay was used to assess the frequency with which host bone marrow cells are retained after marrow grafting. The majority of patients (74%) showed the presence of both host and donor marrow cells when assayed 14 days after transplant. By 84 days posttransplant only 4% of the patients retained host marrow cells. Only 1 of 19 evaluable patients analyzed over 1 year posttransplant showed minimal retention of host cells. No statistical correlation was found between retention of host cells posttransplant and the development of relapse or acute or chronic graft-versus-host disease. Pretransplant conditioning regimen, HLA-matching, diagnosis, disease status at transplant, ABO-matching, and patient age also showed no correlation with the retention of host cells posttransplant.
采用Y染色体特异性原位杂交分析来评估骨髓移植后宿主骨髓细胞留存的频率。多数患者（74%）在移植后14天进行检测时显示同时存在宿主和供体骨髓细胞。移植后84天时，仅有4%的患者留存有宿主骨髓细胞。在移植后1年以上进行分析的19例可评估患者中，只有1例显示宿主细胞有极少留存。未发现移植后宿主细胞的留存与复发、急性或慢性移植物抗宿主病的发生之间存在统计学关联。移植前预处理方案、人类白细胞抗原匹配、诊断、移植时的疾病状态、ABO血型匹配以及患者年龄与移植后宿主细胞的留存也均无关联。
