Przepiorka D, Thomas E D, Durnam D M, Fisher L
Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington.
Am J Clin Pathol. 1991 Feb;95(2):201-6. doi: 10.1093/ajcp/95.2.201.
In situ hybridization for the Y chromosome (Y-ISH) was used to identify residual host cells in the peripheral blood of 51 recipients of sex-mismatched allogeneic marrow not depleted of T cells following conditioning with high-dose cyclophosphamide and total body irradiation (TBI). One patient who rejected the graft showed rapid reappearance of host cells after transient donor marrow engraftment. Host cells were detected at low levels in 49 of the remaining 50 patients. Host peripheral blood mononuclear cells (PBMC) decreased with time and reached a plateau at 1.0 +/- 0.2% within four weeks after transplantation, while the percentage of host granulocytes (GRAN) reached a plateau at background level. The mean absolute numbers of host PBMC or GRAN were less than 0.015 x 10(9)/L and did not vary significantly over the period studied. Neither the percentages nor the absolute numbers of host PBMC or GRAN were significantly affected by HLA-matching, TBI dose-intensity, pretransplant remission status, subsequent development of acute or chronic graft-versus-host disease or relapse after transplantation. The authors conclude that it is common to find a few residual host cells in the peripheral blood of allogeneic marrow transplant recipients, and the presence of these cells has no clinical significance.
采用Y染色体原位杂交技术（Y-ISH），对51例接受了大剂量环磷酰胺和全身照射（TBI）预处理且未进行T细胞清除的性不相合异基因骨髓移植受者外周血中的残留宿主细胞进行鉴定。1例发生移植物排斥反应的患者在短暂的供体骨髓植入后，宿主细胞迅速再次出现。在其余50例患者中，有49例检测到低水平的宿主细胞。宿主外周血单个核细胞（PBMC）随时间减少，在移植后4周内降至1.0±0.2%的平台期，而宿主粒细胞（GRAN）的百分比则降至背景水平的平台期。宿主PBMC或GRAN的平均绝对数量小于0.015×10⁹/L，在研究期间无显著变化。宿主PBMC或GRAN的百分比及绝对数量均未受到HLA配型、TBI剂量强度、移植前缓解状态、随后发生的急性或慢性移植物抗宿主病或移植后复发的显著影响。作者得出结论，在异基因骨髓移植受者外周血中发现少量残留宿主细胞很常见，且这些细胞的存在无临床意义。
